NASC

NASC ID: N538754

Name: SALK_038754

ABRC stock number: SALK_038754

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_038754.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G33700.1 (at chr 1 pos 12212432 (W/12212375-12212432) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 55-79 bps onto the T-DNA. Pools P 9, R 7, C 2 N 8. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO404835.

SALKseq_038754.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G28070.1=UTR3 and AT1G28080.1 (at chr 1 pos 9789256 (C/9789256-9789312) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 9, R 7, C 2 N 8. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO408176.

SALKseq_038754.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G25355.1 (at chr 2 pos 10802485 (W/10802426-10802485) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 55-79 bps onto the T-DNA. Pools P 9, R 7, C 2 N 8. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO411524.

SALKseq_038754.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G14640.1 (around chr 3 pos 4920591 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO414868.

SALKseq_038754.6 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G56300.1 (at chr 5 pos 22799290 (C/22799290-22799341) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 50-79 bps onto the T-DNA. Pools P 9, R 7, C 2 N 8. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO344625.

Donation Date: 2002-01-01

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_038754

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662160 (SALK_038754C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G28070 AT1G28080 AT1G33700 AT2G25355 AT3G14640 AT5G56300 At3g14690

EMBL accession:

ED583751

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At3g14690

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.