NASC

NASC ID: N539714

Name: SALK_039714

ABRC stock number: SALK_039714

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_039714.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G65110.1 (at chr 5 pos 26012723 (W/26012668-26012723) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 14, R 11, C 12 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO384843.

SALKseq_039714.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G13851=P355 and AT2G13860.1 (at chr 2 pos 5807766 (W/5807709-5807766) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 14, R 11, C 12 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO378148.

SALKseq_039714.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G06547.1 (at chr 4 pos 3407553 (W/3407497-3407553) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 14, R 11, C 12 N 19. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO381491.

Donation Date: 2002-01-01

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_039714

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662180 (SALK_039714C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G13851 AT2G13860 AT4G06547 At5g65110

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g65110

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.