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NASC ID: N547519

Name: SALK_047519

ABRC stock number: SALK_047519

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_047519.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G17420.1 (at chr 3 pos 5962185 (W/5962131-5962185) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 16, R 19, C 16 N 20. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO341697.

SALKseq_047519.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G49930.1 and AT1G49938.1 (at chr 1 pos 18486742 (W/18486710-18486742) on the TAIR10). The sequences' 1-33 bps mapped on the genome, 32-59 bps onto the T-DNA. Pools P 16, R 19, C 16 N 20. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO411951.

SALKseq_047519.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G19400.1 (at chr 3 pos 6725272 (W/6725214-6725272) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 16, R 19, C 16 N 20. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO345051.

SALKseq_047519.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G25490.1 (at chr 4 pos 13022958 (W/13022906-13022958) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 16, R 19, C 16 N 20. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO348398.

Donation Date: 2002-01-02

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_047519

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_047519C.

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
  • GPK1
EMBL accession:

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.