NASC Stock Detail Page
NASC ID: N578575
Name: SALK_078575
ABRC stock number: SALK_078575
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. July 2002 TAIR gene annotation - At2g38680. T6A23.12 R300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_078575.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT2G38680.1 (at chr 2 pos 16176013 (W/16175951-16176013) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 65, R 64, C 69 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO430262. SALKseq_078575.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G64790.1 (at chr 1 pos 24070667 (C/24070667-24070712) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 65, R 64, C 69 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO423630. SALKseq_078575.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G18670.1 (at chr 1 pos 6430407 (C/6430407-6430459) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 65, R 64, C 69 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO426946. SALKseq_078575.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT3G53560.1 (at chr 3 pos 19859821 (W/19859766-19859821) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 65, R 64, C 69 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO433568.
Donation Date: 2002-01-07
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_078575
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_078575C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.