NASC

NASC ID: N580893

Name: SALK_080893

ABRC stock number: SALK_080893

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_080893.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G15240.2 (at chr 3 pos 5134347 (W/5134265-5134347) on the TAIR10). The sequences' 1-83 bps mapped on the genome, 82-100 bps onto the T-DNA. Pools P 72, R 74, C 71 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO423999.

SALKseq_080893.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G59100.1 and AT3G59110.1=P355 (at chr 3 pos 21853967 (C/21853967-21854024) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 72, R 74, C 71 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO420683.

SALKseq_080893.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G27270.1 (at chr 4 pos 13663476 (W/13663399-13663476) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 72, R 74, C 71 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO427315.

Donation Date: 2003-01-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_080893

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_080893C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			At3g15240 AT3G15240 AT3G59100 AT3G59110 AT4G27270 At3g15230

EMBL accession:

BZ761741

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.