NASC

NASC ID: N587323

Name: SALK_087323

ABRC stock number: SALK_087323

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

December 2002 TAIR gene annotation - At4g17690 FCAALL.96 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_087323.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G17690.1 (at chr 4 pos 9846614 (C/9846614-9846663) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 75, R 71, C 80 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO438453.

SALKseq_087323.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G10540.1 (at chr 3 pos 3290376 (W/3290312-3290376) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 75, R 71, C 80 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO431842.

SALKseq_087323.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G15250.1=P355 and AT4G15258 (at chr 4 pos 8712005 (C/8712005-8712060) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 75, R 71, C 80 N 75. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO435149.

Donation Date: 2003-01-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_087323

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_087323C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G10540 AT4G15250 AT4G15258 At4g17690

EMBL accession:

BZ595515

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.