NASC Stock Detail Page
NASC ID: N588198
Name: SALK_088198
ABRC stock number: SALK_088198
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. August 2002 TAIR gene annotation - MHK10.18 L300-3', At2g42460. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_088198.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G42450.1=P355 and AT2G42460.1 (at chr 2 pos 17676245 (W/17676168-17676245) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 74, R 71, C 74 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO438612. SALKseq_088198.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G16520.1 (at chr 1 pos 5649814 (W/5649753-5649814) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 74, R 71, C 74 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO432001. SALKseq_088198.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G24530.1=UTR3 and AT1G24540.1 (at chr 1 pos 8699133 (W/8699080-8699133) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 53-78 bps onto the T-DNA. Pools P 74, R 71, C 74 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO435308. SALKseq_088198.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G27950.1=UTR3 and AT5G27960.1 (at chr 5 pos 9989977 (W/9989928-9989977) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 56-78 bps onto the T-DNA. Pools P 74, R 71, C 74 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO418739.
Donation Date: 2002-01-09
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_088198
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_088198C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.