NASC

NASC ID: N589105

Name: SALK_089105

ABRC stock number: SALK_089105

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_089105.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G43573.1 (at chr 3 pos 15487003 (W/15486958-15487003) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 54-78 bps onto the T-DNA. Pools P 74, R 79, C 75 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO422222.

SALKseq_089105.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G44700.1 and AT3G44704.1 (at chr 3 pos 16242300 (C/16242300-16242361) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 74, R 79, C 75 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO425539.

SALKseq_089105.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G21340.1=UTR3 and AT4G21350.1 (at chr 4 pos 11355211 (W/11355154-11355211) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 74, R 79, C 75 N 78. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO428853.

Donation Date: 2003-01-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_089105

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_089105C.

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			At3g43573 AT3G44700 AT3G44704 AT4G21340 AT4G21350

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.