NASC

NASC ID: N626011

Name: SALK_126011

ABRC stock number: SALK_126011

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

January 2003 TAIR gene annotation - At5g62580 K19B1.19 R300-3' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_126011.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G62580.1 (at chr 5 pos 25122430 (W/25122352-25122430) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 55, R 60, C 55 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO360200.

SALKseq_126011.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G64280.1=P355 and AT1G64290.1=UTR3 (at chr 1 pos 23856600 (W/23856522-23856600) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 55, R 60, C 55 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO346828.

SALKseq_126011.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G45070.4=P355 and AT2G45080.1=P355 (at chr 2 pos 18590227 (C/18590227-18590274) on the TAIR10). The sequences' 1-48 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 55, R 60, C 55 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO350173.

SALKseq_126011.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G18150.1 (at chr 3 pos 6218557 (W/6218501-6218557) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 55, R 60, C 55 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO353523.

SALKseq_126011.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G39070.1=P355 and AT4G39080.1=P355 (at chr 4 pos 18208692 (W/18208614-18208692) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 55, R 60, C 55 N 58. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO356848.

Donation Date: 2003-01-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_126011

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_126011C.

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G64280 AT1G64290 AT2G45070 AT2G45080 AT3G18150 AT4G39070 AT4G39080 At5g62580

EMBL accession:

BZ354912

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.