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NASC ID: N626606

Name: SALK_126606

ABRC stock number: SALK_126606

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

January 2003 TAIR gene annotation - At2g21570 At2g21570 R300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_126606.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G03620.1 (at chr 3 pos 874066 (C/874066-874123) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 55-79 bps onto the T-DNA. Pools P 57, R 56, C 52 N 59. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO397051.

SALKseq_126606.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G09080.1 (at chr 4 pos 5803722 (C/5803722-5803771) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 50-79 bps onto the T-DNA. Pools P 57, R 56, C 52 N 59. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO400397.

SALKseq_126606.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G11630.1=UTR3 and AT4G11640.1 (at chr 4 pos 7021454 (W/7021407-7021454) on the TAIR10). The sequences' 1-48 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 57, R 56, C 52 N 59. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO403736.

SALKseq_126606.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G43518.1=UTR3 and AT5G43520.1 (at chr 5 pos 17485209 (W/17485163-17485209) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 55-79 bps onto the T-DNA. Pools P 57, R 56, C 52 N 59. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO407075.

Donation Date: 2003-01-31

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_126606

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_126606C.

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.