NASC

NASC ID: N635247

Name: SALK_135247

ABRC stock number: SALK_135247

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At4g19710 T16H5.7 0 Intron T16H5.7 0 Intron. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_135247.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G19710.1 (at chr 4 pos 10728814 (W/10728759-10728814) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 62, R 65, C 68 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO400805.

SALKseq_135247.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G19420.1=P355 and AT4G19430.1=UTR3 (at chr 4 pos 10591251 (C/10591251-10591328) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 62, R 65, C 68 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO397460.

SALKseq_135247.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G20260.6=UTR3 and AT4G20270.1=P355 (at chr 4 pos 10946240 (C/10946240-10946317) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 62, R 65, C 68 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO404142.

SALKseq_135247.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G19330.1 (at chr 5 pos 6508273 (W/6508226-6508273) on the TAIR10). The sequences' 1-48 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 62, R 65, C 68 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO407475.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_135247

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_135247C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT4G19420 AT4G19430 AT4G20260 AT4G20270 AT5G19330 At4g19710

EMBL accession:

BZ384232

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.