NASC Stock Detail Page
NASC ID: N635272
Name: SALK_135272
ABRC stock number: SALK_135272
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_135272.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G44562 (at chr 5 pos 17948647 (W/17948570-17948647) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 46, R 43, C 44 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO387426. SALKseq_135272.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G04500.1 (at chr 2 pos 1569335 (W/1569284-1569335) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 52-74 bps onto the T-DNA. Pools P 46, R 43, C 44 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO380735. SALKseq_135272.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G36990=P355 and AT5G37000.1 (at chr 5 pos 14617119 (W/14617032-14617119) on the TAIR10). The sequences' 1-89 bps mapped on the genome, 74-95 bps onto the T-DNA. Pools P 46, R 43, C 44 N 47. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO384079.
Donation Date: 2003-03-11
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_135272
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_135272C.
| Status | Price (£) |
|---|---|
| Available to order at NASC | £10.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.