NASC

NASC ID: N636915

Name: SALK_136915

ABRC stock number: SALK_136915

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At5g58480 MQJ2.10 E xon MQJ2.10 E xon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_136915.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G58480.1 (at chr 5 pos 23642066 (W/23641989-23642066) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 77, R 80, C 76 N 77. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO400875.

SALKseq_136915.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G17150.2=P355 and AT2G17160.1 (at chr 2 pos 7472378 (W/7472301-7472378) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 77, R 80, C 76 N 77. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO394190.

SALKseq_136915.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G23110.1 (at chr 5 pos 7774638 (C/7774638-7774689) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 77, R 80, C 76 N 77. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO404211.

SALKseq_136915.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G13850.1 (at chr 4 pos 8020726 (C/8020726-8020805) on the TAIR10). The sequences' 1-80 bps mapped on the genome, 77-95 bps onto the T-DNA. Pools P 77, R 80, C 76 N 77. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO397531.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_136915

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_136915C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G17150 AT2G17160 AT4G13850 AT5G23110 At5g58480

EMBL accession:

BZ766201

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.