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NASC

The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N65907

Name: bob1-1/+

ABRC stock number: CS65907

Description: The STM:GUS construct was transformed into Ws and backcrossed two times to Ler. This STM-GUS reporter line was mutagenized by EMS. The screen for embryo arrest mutants was performed by visualization of GUS in dissected M2 embryos.

Donation Date: 2010-03-26

Donated by:  University of Wisconsin, Madison Kathy Barton

Donor Number: bob1-1/+

Stock type: individual line

Material type: seed

Comment: Donors: Kathy Barton.

Status Price (£)
Available £11.00

Germplasm Info

Seed type: Mutant

Mutagen: ethylmethane sulfonate

Background: Ws

Pedigree: The STM:GUS construct was transformed into Ws and backcrossed two times to Ler.

Growth requirement: none

Phenotype

Segregates 25% embryo lethal homozygous bob1 mutants. Embryo arrest occurs at the globular stage of embryogenesis.

References

Jurkuta et al. 2009. Partitioning the apical domain of the Arabidopsis embryo requires the BOBBER1 NudC domain protein. Plant Cell 21(7):1957-71.PubMed ID: 19648297.