NASC Stock Detail Page
NASC ID: N69102
Name: pRBCS:HF-GFP-RPL18
ABRC stock number: CS69102
Description: The RBSC promoter sequence was cloned into pENTR/D-TOPO. The TMV omega leader sequence, His6-FLAG tag, sGFP, coding region of RPL18B and 3 UTR of the OSC gene were cloned into a modified pPZP111 to form the vector construct, pGATA:HF-sGFP-RPL18. Following recombination, the Col-0 plant was transformed using the floral dip method; kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).
Donation Date: 2015-12-17
| Donated by: | University of California, Riverside Julia Bailey-Serres |
Donor Number: pRBCS:HF-GFP-RPL18
Stock type: individual line
Material type: seed
Comment: Donors: Julia Bailey-Serres. Clone: pRBCS:HF-GFP-RPL18.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Background: Col(Columbia)
Growth requirement: none
Phenotype
No visible phenotype.
References
Mustroph et al. 2009. Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America 106(44):18843-8. PubMed ID: 19843695