NASC

NASC ID: N6946

Name: TILTED 1

ABRC stock number: CS6946

Description: A line homozygous for the shoot apical meristem-expressed PSTM:GUS reporter (the promoter of the SHOOT MERISTEMLESS gene fused to §-glucuronidase) was mutagenized with ethyl methanesulfonate and screened for embryo-defective mutants; backcrossed 3 times to Col.

Donation Date: 2006-10-16

Donated by:

Pennsylvania State University Sally Assmann University of Missouri John Walker Franklin and Marshall College Pablo Jenik

Donor Number: TIL1 / til1-4

Stock type: individual line

Material type: seed

Comment: recessive

Status	Price (£)
Available	£11.00

Germplasm Info

Seed type: Molecular Mapping

Mutagen: ethylmethane sulfonate

Background: WS / Ler

Segregation status: segregating

Pedigree: Backcrossed 3 times to Col.

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	til1-4		At1g08260

Phenotype

Weak allele. Embryos have altered placement of the root pole, slow embryonic development, longer cell cycles (~35%) and larger cells. Abnormal division of the hypophysis in the developing root of the embryo, generating a lens cell that is abnormal in shape or abnormally positioned; asymmetric development of the provasculature, this ultimately leads to an abnormal root pole which is displaced laterally causing the root-shoot axis to be at an angle to the long axis of the suspensor, giving the embryo a tilted appearance. Embryos are viable, have larger cells and nuclei than wild-type embryos but the same DNA content, mutant embryos are larger than wild-type embryos at the same developmental stage. Plants have slow growing roots, slightly delayed flowering, altered floral phyllotaxis, reduced number of ovules, abnormally developing ovules, and reduced fertility.

References

Jenik, P.D., Jurkuta, R.E. & Barton, M.K. 2005. Interactions between the cell cycle and embryonic patterning in Arabidopsis uncovered by a mutation in DNA polymerase epsilon. The Plant Cell 17(12):3362-77.PubMed ID: 16278345.