NASC Stock Detail Page
NASC ID: N799895
Name: CYCLIN A3;3
ABRC stock number: CS799895
Description: Part of transgenic lines carrying reporter constructs for A. thaliana mitotic cyclins. Each line harbors a translational fusion of mitotic cyclin with the GUS reporter gene. These lines were generated by vacuum infiltration of Col-0 plants with Agrobacterium tumefaciens. The vector employed was pMDC163 (Curtis and Grossniklaus, Plant Physiology October 2003 vol. 133 no. 2 462-469). The constructs consist of an entire cyclin genic sequences including introns and ~2 kb upstream promoter regions fused to the GUS cDNA at the position of the termination codon; 3 prime UTR is derived from the nopaline synthase gene. Approximately 20 independent T1 transgenic plants for each construct were prescreened by histochemical GUS staining of flowers, and progeny of three plants with the most representative staining pattern were used for in depth expression analyses in T2 and T3. A seeds of T4 generation of one representative line are in the distributed stocks.
Donation Date: 2013-07-10
| Donated by: | Austrian Academy of Sciences Petra Bulankova Austrian Academy of Sciences Svetlana Akimcheva Austrian Academy of Sciences Karel Riha |
Donor Number: CYCA3;3:GUS
Part of : Bulankova, Akimcheva, Riha CYC:GUS
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col 0
Segregation status: homozygous
Growth requirement: Hygromycin resistance
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
|---|---|---|---|
|
|
|
|
Phenotype
No visible phenotype
References
Bulankova, P. et al. 2013. Identification of Arabidopsis meiotic cyclins reveals functional diversification among plant cyclin genes. PLoS Genetics 9(5):e1003508. PubMed ID: 23671425.