NASC Stock Detail Page
NASC ID: N85243
Name: Tilling line
ABRC stock number: CS85243
Description: Individual line descended from individual M2 plant from ethyl-methane sulfonate (EMS) mutagenesis. Individual lines should be ordered from this population as followup to positive results in the TILLING (Targeting Induced Local Lesions IN Genomes) procedure conducted by the Henikoff laboratory. The reverse genetics, TILLING procedure employs methods which detect heteroduplex DNA for targeted DNA sequences so that EMS-induced point mutations in specific genes of interest can be identified. Details of EMS treatment, screening strategy are provided in the references associated with this resource. All lines are M3 generation, distribution seeds are original donation.
Donation Date: 2001-10-06
| Donated by: | Fred Hutchinson Cancer Research Center Steve Henikoff University of California, Davis Luca Comai |
Donor Number: 2058
Part of : TILLING
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available to order at NASC | £11.00 |
Germplasm Info
Seed type: Point mutation - Tilling
Mutagen: ethylmethane sulfonate
Background: Col-0 (Columbia) with er-105 (erecta mutation) (N89504)
Growth requirement: none
References
Torii, K.U. et al. 1996.The Arabidopsis ERECTA gene encodes a putative receptor protein kinase with extracellular leucine-rich repeats. The Plant Cell 8(4):735-46.PubMed ID: 8624444.