NASC Stock Detail Page
NASC ID: N8530
Name: fedoroff T-DNA line
ABRC stock number: CS8530
Description: Transposon T-DNA line: carries a single copy transposon T-DNA (Ds-GUS T-DNA); the transposon is derived from McClintock's maize Activator (Ac) transposable element; utilizes a beta-glucuronidase (GUS) gene in a promoter/enhancer trap construct; bacterial aph4 gene (hygromycin resistance) is included as a selectable marker; the transposon is inserted in an herbicide-resistant acetolactate synthase gene, whose function is restored when the transposon excises; map location not known; seeds are the R3 generation from a single, homozygous, self-pollinated R2 plant; please consult the Plant Transposon Lab web site for a map of the Ds-GUS T-DNA insertions, information on the use of these stocks and results from transposition experiments; to be crossed to Ac stocks N8533-N8538 to induce transposition.
Donation Date: 1996-08-26
| Donated by: | Pennsylvania State University Nina Fedoroff |
Donor Number: Ds6 393-21
Part of : Fedoroff T-DNA collection
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Enhancer trap
Mutagen: transposon insertion
Background: No-0(N3081)
Transformation method: Agrobacterium transformation
References
Smith, D. et al. 1996. Characterization and mapping of Ds-GUS-T-DNA lines for targeted insertional mutagenesis. The Plant journal 10(4):721-32.PubMed ID: 8893547.