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NASC Stock Detail Page

NASC ID: N869189

Name: SALK_106633.0

ABRC stock number: CS869189

Description: confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.

Donation Date: 2014-04-28

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALKseq_106633.0

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Donors: Joseph R. Ecker. 2015-02-18 GenBank annotation - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G45280.1 (chr 5 pos 18347879 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. GenBank Acc: KG788402

Status Price (£)
Available £10.00

Germplasm Info

Background: Col-0(SALK_106633)

Segregation status: homozygous

Growth requirement: none