NASC

NASC ID: N9727

Name: 35S:LFY

ABRC stock number: CS9727

Description: These transgenic plants constitutively express the flower-meristem-identity gene LEAFY. Dominant early flowering, segregates 3:1 for transgeneSingle locus segregating overexpression of LFY sDNA from 35S promoter, primary vector pCGN18 (Jack, T., Fox, G.L., and Meyerowitz, E.M. 1994. Arabidopsis homeotic gene APETALA3 ectopic expression: transcriptional and posttranscriptional regulation determine organ identity. Cell 76: 703-716), derived from pCGN1547 (McBride, K.E. and Summerfelt, K.R. 1990). Improved binary vectors for Agrobacterium-mediated plant transformation (Plant Mol Biol 14: 269-276.); name of 35S:LFY construct:pDW151

Donation Date: 2007-05-15

Donated by:

Max-Planck-Institute for Developmental Biology Detlef Weigel

Donor Number: DW151.2.5C

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Seed type: Transgenic

Mutagen: ema

Background: Col-7

Segregation status: segregating

Pedigree: Ti was backcrossed twice to Col-7

Marker used: kanamycin

Growth requirement: propagate in short days as segregating stock

Segregation: Dominant early flowering, segregates 3:1

Transformation method: agrobacterium tumefaciens dip

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	lfy	lfy	AT5G61850

Phenotype

Terminal flowers on main inflorescence. Side shoot replaced by solitary flowers. Early-flowering. Reduced fertility. Propagate in short days for best seed set.

References

Weigel, D. & Nilsson, O. 1995. A developmental switch sufficient for flower initiation in diverse plants. Nature 377(6549):495-500.PubMed ID: 7566146.

Nilsson, O., et al. 1998. Flowering-time genes modulate the response to LEAFY activity. Genetics 150(1) 403-410.PubMed ID: 9725856.