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The European Arabidopsis Stock Centre

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NASC ID: N73779

Name: rkfdelta-1 nilr2rir1delta-1 lik1-5

ABRC stock number: CS73779

Description: rkfdelta-1 nilr2rir1delta-1 lik1-5 triple mutant; all three mutations were generated by CRISPR/Cas9 resulting in a 25 kb deletion spanning four genes (At1g29720, At1g29730, At1g29740, and At1g29750) in rkfdelta-1, a 14.9 kb deletion spanning 2 genes (At1g53430 and At1g53440) in nilr2rir1delta-1, and a 5 kb deletion spanning At3g14840 in lik1-5.

Donation Date: 2025-02-27

Donated by:  University of Toronto Daphne Goring

Stock type: individual line

Material type: seed

Status Price (£)
Available £11.00

Germplasm Info

Genus: Arabidopsis     Species: thaliana

Seed type: Transgenic

Mutagen: CRISPR

Background: Col (Columbia)

Segregation status: homozygous

Growth requirement: none

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
  • rkfdelta-1
  • nilr2rir1delta-1
  • lik1-5

Phenotype

Wild type pollen grains hydrate more slowly on mutant stigmas, callose plugs in pollen tubes are shorter and wider, C. rubella pollen adheres to the stigma more effectively and more pollen tubes were observed growing in mutant styles.

References

Lee HK, Canales Sanchez LE, Bordeleau SJ, Goring DR.; 2024-04-30; Arabidopsis leucine-rich repeat malectin receptor-like kinases regulate pollen-stigma interactions. ; Plant Physiol.; PubMed ID: 38270530