Stock Solutions: (use double distilled water for all solutions, stocks are stable at 4C) stock conc. 1 KNO3 1.2 M 12 g / 100 ml MgSO4.7H2O 80 mM 1.97 g / 100 ml 2 Ca(NO3)2.4H2O 30% 30 g / 100 ml 3 KH2PO4 1 M 13.6 g / 100 ml 4 FeNaEDTA 20 mM 0.73 g / 100 ml 5 Micronutrients see below Micronutrients (all in mg / 100 ml stock solution) H3BO3 286 MnCl2 181 CuCl2.2H2O 5 ZnSO4 20 CoCl2.6H2O 3 Na2MoO4.2H2O 7 NaCl 585 To prepare 1 liter germination medium; add to distilled water: 2.5 ml stock 1 1 ml stock 2 0.5 ml stock 3 0.5 ml stock 4 0.25 ml stock 5, adjust pH to 6.4 with 1N KOH (ca. 125 microlitre / l medium) add 6g agar / liter (preferably tissue culture quality)Reference: