Promoter tagging construct pMOG553

pMOG553 was constructed as follows: a gusA intron gene linked to a 35S CaMV terminator (Vancanneyt et al. 1990 Mol. Gen. Genet. 220;245-250) was fused to synthetic oligomers harbouring the octopine right border and octopine overdrive sequences from which all stop codons were removed. This cassette was transferred as an EcoRI-BglII fragment into pMOG22 (Goddijn et al Plant J.4, 863-873)

The complete sequence for the entire BglII fragment will be available early 1995 through the Nottingham Stock Centre and EMBL. The sequence around the right border is: 

5' TAG ATC TGG GGA ACC CTG TGG TTG GCA TGC CAA ACA AAC ATA CAC AGC GAC TTA 
   1                        20                         40

ATC GAT TTA CAA CGG TAT ATA TCC TGC CAA AGC TTG GAT CCC CGG GTA GGT CAG TCC 
      60                         80                         100

CTT ATG 3'
       117
2-7 = Bgl II site
61-85 = octopine right border
115 = start ATG from gusA

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