Sequence Analysis for 168H2T7

Sample Output from University of Minnesota's Sequence Analysis Project

(A collaboration between the University of Minnesota and Michigan State University, funded by NSF)

This page shows only a small portion of the output available from the University of Minnesota's database. For further information regarding this work, go to the University of Minnesota's Computational Biology Centers' Home Page OR Back to the Sample TC Report

Sequence Origin: Michigan State University Arabidopsis cDNA Sequencing Project

Analysis Origin: University of Minnesota Plant Molecular Informatics Center

dbEST ID for this sequence: 242025

GenBank Accession Number for this sequence: R65270

Processed Sequence after Filtering and/or Trimming for Quality

Visualization of BLAST Hits

Percentage of Unknown (N) bases in Sequence

Low Complexity Regions

Blastx Results Summary

Blastn Results Summary

Blastx Hits Ordered by P-value

Blastn Hits Ordered by P-value

Citations and credits

Plus Strand Processed

DNA Sequence after Vector Filtering and End Trimming for Quality

(Each Full Line is 40 bp Long)

TCCTCACTTTCATCAGCCGTTTTNAATCTCCGGCGACTTG
ACAGAGAAGAACAAGGAAGAAGACTAAGAGAGAAAGTAAG
AGATAATCCAGGAGATTCATTCTCCGTTTTNAATCTNCCT
CAATCTCATCTTCTTCCGCTCTTNCTTTCCAAGCTCATAA
AAAATGGCTGAGGCTGATGATATTCAACCAATCGTGTGTG
ACAATGGTACCGGTATGGTGAAGGCTGGATTTNCAGGAGA
TGATGCTCCCAGGGCTGTTTTTCCCAGTNTTGTTGGTAGG
CCAAGACATCATGGTGTCATGGTTGGNATGAACCAGAAGG
ATGCATATNTTGGTGATGAAGCACAATCCANGNGAGGTAT
TCTTACCTTGAAGNATCCTTTTTGNGCATGGNGTTTTNAG
CACNNGGGGTG

Sequence Length: 411

Percentage of Unknown (N) bases in Sequence

524 original bp (6.7% unknown bases), reduced to 411 bp (3.9% unknown bases).

Visualization of Blastx Hits from AlignmentViewer Tool

Visualization of Blastp Hits (where Low Complexity Regions have been removed) from AlignmentViewer Tool

Hits in a frame will be displayed if and only if there were low complexity regions within the BLASTX hits for any given frame. Otherwise nothing will be displayed. Lack of hits may be because the low complexity regions were only found in the incorrect frames. In the future the visualization will be improved to eliminate this and improve clarity.

Low Complexity Regions from XNU program

Low complexity regions found for translated frame +1 :

None

Low complexity regions found for translated frame +2 :

None

Low complexity regions found for translated frame +3 :

Translated Protein: start = 3, end = 37

Corresponding DNA : start = 9, end = 113

The function of this Sequence can possibly be determined by the following BLASTX, BLASTN, AND/OR BLASTP hits.

Summary of Blastx Hits on Plus Strand with P-value <= 1.000e-01

BLAST DB SEARCHED: Non-redundant PIR (RELEASE 44.00) plus GenPept, from GenBank (RELEASE 89.0)

DATE SEARCHED: Sat Jul 8 06:38:09 CDT 1995

Click on the "goto" in the first column to see more details: e.g. alignment and longer description of hit sequence from databank.

                          High  
More DB  Acc    Fr Shift? Score P-value  Description
---- --  ---    -- ------ ----- -------  -----------
goto pir  S10020   2     N      292   7.7e-25   actin 1 - rice                                    
goto pir  S47090   2     N      292   7.7e-25   actin - sorghum PEP:gi|499012|gp|X79378|SVSOAC1_1... 
goto  gi  20322    2     N      292   7.7e-25   Oryza sativa RAc1 mRNA for actin. [Oryza sativa]  
goto  gi  295885   2     N      292   7.7e-25   actin [Oryza sativa]                              
goto pir  JA0066   2     N      291   9.9e-25   actin - Arabidopsis thaliana PEP:gi|166582|gp|M2 ... 
goto pir  S31933   2     N      291   9.9e-25   actin - common tobacco PEP:pir|S35257|S35257 acti... 
goto pir  S20098   2     N      291   9.9e-25   actin - potato PEP:gi|21544|gp|X55751|STPOAC97_1 ... 
goto pir  S20093   2     N      290   1.3e-24   actin - potato PEP:gi|21534|gp|X55752|STPOAC101_1... 
goto pir  S20094   2     N      290   1.3e-24   actin - potato PEP:gi|21536|gp|X55749|STPOAC58_1 ... 
goto  gi  553858   2     N      272   2.0e-24   alpha-cardiac actin [Mus musculus]

Summary of Blastn Hits on Plus Strand with P-value <= 1.000e-01

BLAST DB SEARCHED: From NCBI- Non-redundant GenBank (RELEASE 89.0)

DATE SEARCHED: Sat Jul 8 06:40:48 CDT 1995

Click on the "goto" in the first column to see more details: e.g. alignment and longer description of hit sequence from databank.

More DB  Acc    Strand Score P-value  Description
---- --  ---    ------ ----- -------  -----------
goto  emb Z25952    Plus   1620   1.1e-129  A. thaliana transcribed sequence; clone VBV24-599 ... 
goto   gb R29797    Plus   1572   9.1e-126  12402 Arabidopsis thaliana cDNA clone 161E2T7.    
goto   gb T46735    Plus   1567   1.6e-125  9998 Arabidopsis thaliana cDNA clone 141M5T7.     
goto   gb R30501    Plus   1516   3.4e-121  13106 Arabidopsis thaliana cDNA clone 164G21T7.   
goto   gb T21821    Plus   1507   2.2e-120  3829 Arabidopsis thaliana cDNA clone 103C21T7.    
goto   gb T76037    Plus   1457   2.7e-116  10815 Arabidopsis thaliana cDNA clone 140H23T7.   
goto   gb T43759    Plus   1350   2.6e-107  7022 Arabidopsis thaliana cDNA clone 123C3T7.     
goto   gb T20889    Plus    890   7.1e-69   2897 Arabidopsis thaliana cDNA clone 89C9T7.      
goto  emb Z37638    Plus    782   4.6e-60   A. thaliana transcribed sequence; clone VBVHC11;  ... 
goto  emb X17526    Plus    698   2.5e-49   D.carota RNA for actin gene Ac1.

BLASTX Hits by P-value

Highest P-value cutoff for reporting hits = 0.100

Hit Accession Number S10020 of PIR database

Hit Sequence Description :: actin 1 - rice
Length of Hit Sequence = 377
P-value = 7.700000e-25

HSP Alignments:

Score: 292 Frame = 2 Identities = 83% Positives = 89%

164 MAEADDIQPIVCDNGTGMVKAGFXGDDAPRAVFPSXVGRPRHHGVMVGMNQKDAYXGDEA 343

MA+A+DIQP+VCDNGTGMVKAGF GDDAPRAVFPS VGRPRH GVMVGM QKDAY GDEA

1 MADAEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEA 60

344 QSXXGILTLKXPF 382

QS GILTLK P+

1 QSKRGILTLKYPI 73

____________________________

Hit Accession Number S47090 of PIR database

Hit Sequence Description :: actin - sorghumPEP_:gi|499012|gp|X79378|SVSOAC1_1 actin [Sorghum bicolor]
Length of Hit Sequence = 377
P-value = 7.700000e-25

HSP Alignments:

Score: 292 Frame = 2 Identities = 83% Positives = 89%

164 MAEADDIQPIVCDNGTGMVKAGFXGDDAPRAVFPSXVGRPRHHGVMVGMNQKDAYXGDEA 343

MA+A+DIQP+VCDNGTGMVKAGF GDDAPRAVFPS VGRPRH GVMVGM QKDAY GDEA

1 MADAEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEA 60

344 QSXXGILTLKXPF 382

QS GILTLK P+

1 QSKRGILTLKYPI 73

____________________________

Hit Accession Number 20322 of GenInfo database

Hit Sequence Description :: Oryza sativa RAc1 mRNA for actin. [Oryza sativa]
Length of Hit Sequence = 377
P-value = 7.700000e-25

HSP Alignments:

Score: 292 Frame = 2 Identities = 83% Positives = 89%

164 MAEADDIQPIVCDNGTGMVKAGFXGDDAPRAVFPSXVGRPRHHGVMVGMNQKDAYXGDEA 343

MA+A+DIQP+VCDNGTGMVKAGF GDDAPRAVFPS VGRPRH GVMVGM QKDAY GDEA

1 MADAEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEA 60

344 QSXXGILTLKXPF 382

QS GILTLK P+

1 QSKRGILTLKYPI 73

____________________________

Hit Accession Number 295885 of GenInfo database

Hit Sequence Description :: actin [Oryza sativa]
Length of Hit Sequence = 377
P-value = 7.700000e-25

HSP Alignments:

Score: 292 Frame = 2 Identities = 83% Positives = 89%

164 MAEADDIQPIVCDNGTGMVKAGFXGDDAPRAVFPSXVGRPRHHGVMVGMNQKDAYXGDEA 343

MA+A+DIQP+VCDNGTGMVKAGF GDDAPRAVFPS VGRPRH GVMVGM QKDAY GDEA

1 MADAEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEA 60

344 QSXXGILTLKXPF 382

QS GILTLK P+

1 QSKRGILTLKYPI 73

____________________________

Hit Accession Number JA0066 of PIR database

Hit Sequence Description :: actin - Arabidopsis thalianaPEP_:gi|166582|gp|M20016|ATHACT1A_1 A.thaliana AAc1 gene encoding actin-1, complete cds. [Arabidopsis thaliana]
Length of Hit Sequence = 377
P-value = 9.900000e-25

HSP Alignments:

Score: 291 Frame = 2 Identities = 82% Positives = 89%

164 MAEADDIQPIVCDNGTGMVKAGFXGDDAPRAVFPSXVGRPRHHGVMVGMNQKDAYXGDEA 343

MA+++DIQP+VCDNGTGMVKAGF GDDAPRAVFPS VGRPRH GVMVGM QKDAY GDEA

1 MADGEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEA 60

344 QSXXGILTLKXPF 382

QS GILTLK P+

1 QSKRGILTLKYPI 73

____________________________

Hit Accession Number S31933 of PIR database

Hit Sequence Description :: actin - common tobaccoPEP_:pir|S35257|S35257 actin - common tobaccoPEP_:gi|22609|gp|X63603|NTACTI_1 actin [Nicotiana tabacum]
Length of Hit Sequence = 377
P-value = 9.900000e-25

HSP Alignments:

Score: 291 Frame = 2 Identities = 82% Positives = 89%

164 MAEADDIQPIVCDNGTGMVKAGFXGDDAPRAVFPSXVGRPRHHGVMVGMNQKDAYXGDEA 343

MA+++DIQP+VCDNGTGMVKAGF GDDAPRAVFPS VGRPRH GVMVGM QKDAY GDEA

1 MADGEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEA 60

344 QSXXGILTLKXPF 382

QS GILTLK P+

1 QSKRGILTLKYPI 73

____________________________

Hit Accession Number S20098 of PIR database

Hit Sequence Description :: actin - potatoPEP_:gi|21544|gp|X55751|STPOAC97_1 actin [Solanum tuberosum]
Length of Hit Sequence = 377
P-value = 9.900000e-25

HSP Alignments:

Score: 291 Frame = 2 Identities = 82% Positives = 89%

164 MAEADDIQPIVCDNGTGMVKAGFXGDDAPRAVFPSXVGRPRHHGVMVGMNQKDAYXGDEA 343

MA+++DIQP+VCDNGTGMVKAGF GDDAPRAVFPS VGRPRH GVMVGM QKDAY GDEA

1 MADGEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHSGVMVGMGQKDAYVGDEA 60

344 QSXXGILTLKXPF 382

QS GILTLK P+

1 QSKRGILTLKYPI 73

____________________________

Hit Accession Number S20093 of PIR database

Hit Sequence Description :: actin - potatoPEP_:gi|21534|gp|X55752|STPOAC101_1 actin [Solanum tuberosum]
Length of Hit Sequence = 377
P-value = 1.300000e-24

HSP Alignments:

Score: 290 Frame = 2 Identities = 82% Positives = 89%

164 MAEADDIQPIVCDNGTGMVKAGFXGDDAPRAVFPSXVGRPRHHGVMVGMNQKDAYXGDEA 343

MA+A+DI+P+VCDNGTGMVKAGF GDDAPRAVFPS VGRPRH GVMVGM QKDAY GDEA

1 MADAEDIEPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEA 60

344 QSXXGILTLKXPF 382

QS GILTLK P+

1 QSKRGILTLKYPI 73

____________________________

Hit Accession Number S20094 of PIR database

Hit Sequence Description :: actin - potatoPEP_:gi|21536|gp|X55749|STPOAC58_1 actin [Solanum tuberosum]
Length of Hit Sequence = 377
P-value = 1.300000e-24

HSP Alignments:

Score: 290 Frame = 2 Identities = 82% Positives = 87%

164 MAEADDIQPIVCDNGTGMVKAGFXGDDAPRAVFPSXVGRPRHHGVMVGMNQKDAYXGDEA 343

MAE++DIQP+VCDNGTGMVKAGF GDDAPRAVFP VGRPRH GVMVGM QKDAY GDEA

1 MAEGEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPRIVGRPRHTGVMVGMGQKDAYVGDEA 60

344 QSXXGILTLKXPF 382

QS GILTLK P+

1 QSKRGILTLKYPI 73

____________________________

Hit Accession Number 553858 of GenInfo database

Hit Sequence Description :: alpha-cardiac actin [Mus musculus]
Length of Hit Sequence = 158
P-value = 2.000000e-24

HSP Alignments:

Score: 272 Frame = 2 Identities = 70% Positives = 85%

161 KMAEADDIQPIVCDNGTGMVKAGFXGDDAPRAVFPSXVGRPRHHGVMVGMNQKDAYXGDE 340

+M + ++ ++VCDNG+G+VKAGF GDDAPRAVFPS VGRPRH+GVMVGM QKD+Y GDE

7 RMCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 66

341 AQSXXGILTLKXPF 382

AQS GILTLK P+

7 AQSKRGILTLKYPI 80

____________________________

BLASTN Hits by P-value

Highest P-value cutoff for reporting hits = 0.100

Hit Accession Number Z25952 originally from EMBL database

Hit Sequence Description :: A. thaliana transcribed sequence; clone VBV24-5992; 5' end; similar to Actin.
Length of Hit Sequence = 352
P-value = 1.100000e-129

HSP Alignments:

 Score: 1620  Strand = Plus   Identities = 95%
   2 CCTCACTTTCATCAGCCGTTTTNAATCTCCGGCGACTTGACAGAGAAGAACAAGGAAGAA 61

     |||||||||||||||||||||| ||||||||||| |||||||||||||||||||||||||

   1 CCTCACTTTCATCAGCCGTTTTGAATCTCCGGCGNCTTGACAGAGAAGAACAAGGAAGAA 60

  62 GACTAAGAGAGAAAGTAAGAGATAATCCAGGAGATTCATTCTCCGTTTTNAATCTNCCTC 121
     |||||||||||||||||||||||||||||||||||||||||||||||||  |||| ||||

  61 GACTAAGAGAGAAAGTAAGAGATAATCCAGGAGATTCATTCTCCGTTTTGNATCTTCCTC 120

 122 AATCTCATCTTCTTCCGCTCTTNCTTTCCAAGCTCATAAAAAATGGCTGAGGCTGATGAT 181
     |||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||

 121 AATCTCATCTTCTTCCGCTCTTTCTTTCCAAGCTCATAAAAAATGGCTGAGGCTGATGAT 180

 182 ATTCAACCAATCGTGTGTGACAATGGTACCGGTATGGTGAAGGCTGGATTTNCAGGAGAT 241
     |||||||||||||||||||||||| ||||| |||||||||||||||||||| ||||||||

 181 ATTCAACCAATCGTGTGTGACAATNGTACCNGTATGGTGAAGGCTGGATTTNCAGGAGAT 240

 242 GATGCTCCCAGGGCTGTTTTTCCCAGTNTTGTTGGTAGGCCAAGACATCATGGTGTCATG 301
     ||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||

 241 GATGCTCCCAGGGCTGTTTTTCCCAGTNTTGTTGGTAGGCCAAGACATCATGGTGTCATG 300

 302 GTTGGNATGAACCAGAAGGATGCATATNTTGGTGATGAAGCACAATCCANG 352
     ||| | ||||||||||||||||||||| ||||| ||||||||||||||| |

 301 GTTNGGATGAACCAGAAGGATGCATATGTTGGTNATGAAGCACAATCCAAG 351

____________________________

Hit Accession Number R29797 of Genbank database

Hit Sequence Description :: 12402 Arabidopsis thaliana cDNA clone 161E2T7.
Length of Hit Sequence = 401
P-value = 9.100000e-126

HSP Alignments:

 Score: 1572  Strand = Plus   Identities = 96%
  19 GTTTTNAATCTCCGGCGACTTGACAGAGAAGAACAAGGAAGAAGACTAAGAGAGAAAGTA 78

     ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||

   2 GTTTTNAATCTCCGGCGACTTGACAGAGAAGAACAAGGAAGAAGACTAAGAGAGAAAGTA 61

  79 AGAGATAATCCAGGAGATTCATTCTCCGTTTTNAATCTNCCTCAATCTCATCTTCTTCCG 138
     |||||||||||||||||||||||||||||||| ||||| |||||||||||||||||||||

  62 AGAGATAATCCAGGAGATTCATTCTCCGTTTTNAATCTNCCTCAATCTCATCTTCTTCCG 121

 139 CTCTTNCTTTCCAAGCTCATAAAAAATGGCTGAGGCTGATGATATTCAACCAATCGTGTG 198
     ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||

 122 CTCTTNCTTTCCAAGCTCATAAAAAATGGCTGAGGCTGATGATATTCAACCAATCGTGTG 181

 199 TGACAATGGTACCGGTATGGTGAAGGCTGGATTTNCAGGAGATGATGCTCCCAGGGCTGT 258
     |||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||

 182 TGACAATGGTACCGGTATGGTGAAGGCTGGATTTNCAGGAGATGATGCTCCCAGGGCTGT 241

 259 TTTTCCCAGTNTTGTTGGTAGGCCAAGACATCATGGTGTCATGGTTGGNATGAACCAGAA 318
     ||| |||||| ||||||||||||||||||||||||||||||||||||| |||||||||||

 242 TTTNCCCAGTGTTGTTGGTAGGCCAAGACATCATGGTGTCATGGTTGGNATGAACCAGAA 301

 319 GGATGCATATNTTGGTGATGAAGCACAATCCANGNG 354
     |||||||||| |||||||||||||||||| || | |

 302 GGATGCATATNTTGGTGATGAAGCACAATNCAAGNG 337

____________________________

Hit Accession Number T46735 of Genbank database

Hit Sequence Description :: 9998 Arabidopsis thaliana cDNA clone 141M5T7.
Length of Hit Sequence = 598
P-value = 1.600000e-125

HSP Alignments:

 Score: 1567  Strand = Plus   Identities = 97%
   3 CTCACTTTCATCAGCCGTTTTNAATCTCCGGCGACTTGACAGAGAAGAACAAGGAAGAAG 62

     ||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||

   2 CTCACTTTCATCAGCCGTTTTGAATCTCCGGCGACTTGACAGAGAAGAACAAGGAAGAAG 61

  63 ACTAAGAGAGAAAGTAAGAGATAATCCAGGAGATTCATTCTCCGTTTTNAATCTNCCTCA 122
     |||||||||||||||||||||||||||||||||||||||||||||||| ||||| |||||

  62 ACTAAGAGAGAAAGTAAGAGATAATCCAGGAGATTCATTCTCCGTTTTGAATCTTCCTCA 121

 123 ATCTCATCTTCTTCCGCTCTTNCTTTCCAAGCTCATAAAAAATGGCTGAGGCTGATGATA 182
     ||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||

 122 ATCTCATCTTCTTCCGCTCTTTCTTTCCAAGCTCATAAAAAATGGCTGAGGCTGATGATA 181

 183 TTCAACCAATCGTGTGTGACAATGGTACCGGTATGGTGAAGGCTGGATTTNCAGGAGATG 242
     |||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||

 182 TTCAACCAATCGTGTGTGACAATGGTACCGGTATGGTGAAGGCTGGATTTGCAGGAGATG 241

 243 ATGCTCCCAGGGCTGTTTTTCCCAGTNTTGTTGGTAGGCCAAGACATCATGGTGTCATGG 302
     |||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||

 242 ATGCTCCCAGGGCTGTTTTTCCCAGTGTTGTTGGTAGGCCAAGACATCATGGTGTCATGG 301

 303 TTGGNATGAACCAGAAGGATGCATAT 328
     |||| |||||||||||||||||||||

 302 TTGGGATGAACCAGAAGGATGCATAT 327

 Score: 165  Strand = Plus   Identities = -1%
 328 TNTTGGTGATGAAGCACAATCCANGNGAGGTATTCTTACCTT 369

     | ||||||||||||||||||||| | |||| ||||||||| |

 328 TGTTGGTGATGAAGCACAATCCAAGAGAGGGATTCTTACCCT 369

____________________________

Hit Accession Number R30501 of Genbank database

Hit Sequence Description :: 13106 Arabidopsis thaliana cDNA clone 164G21T7.
Length of Hit Sequence = 486
P-value = 3.400000e-121

HSP Alignments:

 Score: 1516  Strand = Plus   Identities = 96%
  28 CTCCGGCGACTTGACAGAGAAGAACAAGGAAGAAGACTAAGAGAGAAAGTAAGAGATAAT 87

     ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

   2 CTCCGGCGACTTGACAGAGAAGAACAAGGAAGAAGACTAAGAGAGAAAGTAAGAGATAAT 61

  88 CCAGGAGATTCATTCTCCGTTTTNAATCTNCCTCAATCTCATCTTCTTCCGCTCTTNCTT 147
     ||||||||||||||||||||||| ||||| |||||||||||||||||||||||||| |||

  62 CCAGGAGATTCATTCTCCGTTTTGAATCTNCCTCAATCTCATCTTCTTCCGCTCTTTCTT 121

 148 TCCAAGCTCATAAAAAATGGCTGAGGCTGATGATATTCAACCAATCGTGTGTGACAATGG 207
     ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

 122 TCCAAGCTCATAAAAAATGGCTGAGGCTGATGATATTCAACCAATCGTGTGTGACAATGG 181

 208 TACCGGTATGGTGAAGGCTGGATTTNCAGGAGATGATGCTCCCAGGGCTGTTTTTCCCAG 267
     ||||| ||||||||||||||||||| ||||||||||||||||||||||||||||||||||

 182 TACCGNTATGGTGAAGGCTGGATTTGCAGGAGATGATGCTCCCAGGGCTGTTTTTCCCAG 241

 268 TNTTGTTGGTAGGCCAAGACATCATGGTGTCATGGTTGGNATGAACCAGAAGGATGCATA 327
     | |||||||||||||| |||||||||||||||||||||| ||| |||| |||||||||||

 242 TGTTGTTGGTAGGCCACGACATCATGGTGTCATGGTTGGGATGNACCACAAGGATGCATA 301

 328 TNTTGGTGATGAAGCACAATCCA 350
     | |||||||||||||||||||||

 302 TGTTGGTGATGAAGCACAATCCA 324

 Score: 192  Strand = Plus   Identities = -1%
 352 GNGAGGTATTCTTACCTTGAAGNATCCTTTTTGNGCATGGNGTTTTNAGCACNNGGGGTG 411

     | ||||||||||||||| |||| ||||| |||| |||||| ||| | ||||    |||||

 327 GAGAGGTATTCTTACCTCGAAGTATCCTCTTTGAGCATGGTGTTGTTAGCANNTNGGGTG 386

____________________________

Hit Accession Number T21821 of Genbank database

Hit Sequence Description :: 3829 Arabidopsis thaliana cDNA clone 103C21T7.
Length of Hit Sequence = 426
P-value = 2.200000e-120

HSP Alignments:

 Score: 1507  Strand = Plus   Identities = 96%
  11 CATCAGCCGTTTTNAATCTCCGGCGACTTGACAGAGAAGAACAAGGAAGAAGACTAAGAG 70

     ||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||

   2 CATCAGCCGTTTTNAATCTCCGGCGACTTGACAGAGAAGAACAAGGAAGAAGACTAAGAG 61

  71 AGAAAGTAAGAGATAATCCAGGAGATTCATTCTCCGTTTTNAATCTNCCTCAATCTCATC 130
     ||||||||||| |||||||||||||||||||||||||||| ||||| |||||||||||||

  62 AGAAAGTAAGANATAATCCAGGAGATTCATTCTCCGTTTTNAATCTNCCTCAATCTCATC 121

 131 TTCTTCCGCTCTTNCTTTCCAAGCTCATAAAAAATGGCTGAGGCTGATGATATTCAACCA 190
     ||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||

 122 TTCTTCCGCTCTTTCTTTCCAAGCTCATAAAAAATGGCTGAGGCTGATGATATTCAACCA 181

 191 ATCGTGTGTGACAATGGTACCGGTATGGTGAAGGCTGGATTTNCAGGAGATGATGCTCCC 250
     ||||||||| |||||||||||||||||||||||||||||||| |||||||||||||||||

 182 ATCGTGTGTNACAATGGTACCGGTATGGTGAAGGCTGGATTTNCAGGAGATGATGCTCCC 241

 251 AGGGCTGTTTTTCCCAGTNTTGTTGGTAGGCCAAGACATCATGGTGTCATGGTTGGNATG 310
     |||||||||||||||||| ||||||||||||||||||||||||||||||||||||| |||

 242 AGGGCTGTTTTTCCCAGTNTTGTTGGTAGGCCAAGACATCATGGTGTCATGGTTGGNATG 301

 311 AACCAGAAGGATGCATATNTTGG 333
     |||||||||| ||||||| || |

 302 AACCAGAAGGNTGCATATNTTNG 324

____________________________

Hit Accession Number T76037 of Genbank database

Hit Sequence Description :: 10815 Arabidopsis thaliana cDNA clone 140H23T7.
Length of Hit Sequence = 492
P-value = 2.700000e-116

HSP Alignments:

 Score: 1457  Strand = Plus   Identities = 97%
  28 CTCCGGCGACTTGACAGAGAAGAACAAGGAAGAAGACTAAGAGAGAAAGTAAGAGATAAT 87

     ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

   2 CTCCGGCGACTTGACAGAGAAGAACAAGGAAGAAGACTAAGAGAGAAAGTAAGAGATAAT 61

  88 CCAGGAGATTCATTCTCCGTTTTNAATCTNCCTCAATCTCATCTTCTTCCGCTCTTNCTT 147
     ||||||||||||||||||||||| ||||| |||||||||||||||||||||||||| |||

  62 CCAGGAGATTCATTCTCCGTTTTGAATCTTCCTCAATCTCATCTTCTTCCGCTCTTTCTT 121

 148 TCCAAGCTCATAAAAAATGGCTGAGGCTGATGATATTCAACCAATCGTGTGTGACAATGG 207
     ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

 122 TCCAAGCTCATAAAAAATGGCTGAGGCTGATGATATTCAACCAATCGTGTGTGACAATGG 181

 208 TACCGGTATGGTGAAGGCTGGATTTNCAGGAGATGATGCTCCCAGGGCTGTTTTTCCCAG 267
     ||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||

 182 TACCGGTATGGTGAAGGCTGGATTTGCAGGAGATGATGCTCCCAGGGCTGTTTTTCCCAG 241

 268 TNTTGTTGGTAGGCCAAGACATCATGGTGTCATGGTTGGNATGAACCAGAAGGATGCATA 327
     | ||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||

 242 TGTTGTTGGTAGGCCAAGACATCATGGTGTCATGGTTGGGATGAACCAGAAGGATGCATA 301

 328 TNTT 331
     | ||

 302 TGTT 305

 Score: 168  Strand = Plus   Identities = -1%
 354 GAGGTATTCTTACCTTGAAGNATCCTTTTTGNGCATGGNGTTTTNAGCACNNGGGGT 410

     | ||||||||||||||||||    | | ||| ||||||  |||| |||||  |||||

 331 GGGGTATTCTTACCTTGAAGGTATCCTATTGAGCATGGTTTTTTTAGCACCTGGGGT 387

____________________________

Hit Accession Number T43759 of Genbank database

Hit Sequence Description :: 7022 Arabidopsis thaliana cDNA clone 123C3T7.
Length of Hit Sequence = 419
P-value = 2.600000e-107

HSP Alignments:

 Score: 1350  Strand = Plus   Identities = 96%
   4 TCACTTTCATCAGCCGTTTTNAATCTCCGGCGACTTGACAGAGAAGAACAAGGAAGAAGA 63

     |||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||

   2 TCACTTTCATCAGCCGTTTTGAATCTCCGGCGACTTGACAGAGAAGAACAAGGAAGAAGA 61

  64 CTAAGAGAGAAAGTAAGAGATAATCCAGGAGATTCATTCTCCGTTTTNAATCTNCCTCAA 123
     ||||||||||||||||||||||||||||||||||||||||||||||| ||||| ||||||

  62 CTAAGAGAGAAAGTAAGAGATAATCCAGGAGATTCATTCTCCGTTTTGAATCTTCCTCAA 121

 124 TCTCATCTTCTTCCGCTCTTNCTTTCCAAGCTCATAAAAAATGGCTGAGGCTGATGATAT 183
     |||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||

 122 TCTCATCTTCTTCCGCTCTTTCTTTCCAAGCTCATAAAAAATGGCTGAGGCTGATGATAT 181

 184 TCAACCAATCGTGTGTGACAATGGTACCGGTATGGTGAAGGCTGGATTTNCAGGAGATGA 243
     ||||||||||||||||||||||||||||||||||||||||||||||||| |||| |||||

 182 TCAACCAATCGTGTGTGACAATGGTACCGGTATGGTGAAGGCTGGATTTGCAGGNGATGA 241

 244 TGCTCCCAGGGCTGTTTTTCCCAGTNTTGTTGGTAGGCCAAGACATCA 291
     ||||||||||||||||||| ||||| |||||||||||||||| || ||

 242 TGCTCCCAGGGCTGTTTTTNCCAGTGTTGTTGGTAGGCCAAGNCAACA 289

____________________________

Hit Accession Number T20889 of Genbank database

Hit Sequence Description :: 2897 Arabidopsis thaliana cDNA clone 89C9T7.
Length of Hit Sequence = 277
P-value = 7.100000e-69

HSP Alignments:

 Score: 890  Strand = Plus   Identities = 94%
   2 CCTCACTTTCATCAGCCGTTTTNAATCTCCGGCGACTTGACAGAGAAGAACAAGGAAGAA 61

     |||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||

   2 CCTCACTTTCATCAGCCGTTTTGAATCTCCGGCGACTTGACAGAGAAGAACAAGGAAGAA 61

  62 GACTAAGAGAGAAAGTAAGAGATAATCCAGGAGATTCATTCTCCGTTTTNAATCTNCCTC 121
     ||||||||||||||||||||||||||||||| ||||||||||||||||| ||||| ||||

  62 GACTAAGAGAGAAAGTAAGAGATAATCCAGGNGATTCATTCTCCGTTTTGAATCTTCCTC 121

 122 AATCTCATCTTCTTCCGCTCTTNCTTTCCAAGCTCATAAAAAATGGCTGAGGCTGATGAT 181
     ||||||| |||||| |  |||| |||||||||||||||||||||||||||||||||||||

 122 AATCTCANCTTCTTTCNNTCTTTCTTTCCAAGCTCATAAAAAATGGCTGAGGCTGATGAT 181

 182 ATTCAACCAATCGTGT 197
     ||||||||||||| ||

 182 ATTCAACCAATCGNGT 197

____________________________

Hit Accession Number Z37638 originally from EMBL database

Hit Sequence Description :: A. thaliana transcribed sequence; clone VBVHC11; 5' end; Similar to Actin 1 ; Oryza sativa.
Length of Hit Sequence = 413
P-value = 4.600000e-60

HSP Alignments:

 Score: 782  Strand = Plus   Identities = 81%
 151 AAGCTCATAAAAAATGGCTGAGGCTGATGATATTCAACCAATCGTGTGTGACAATGGTAC 210

     ||| || || |  ||||| || |||||||| |||||| | || || ||||||||||||||

  79 AAGATCGTAGACCATGGCCGATGCTGATGACATTCAANCTATTGTCTGTGACAATGGTAC 138

 211 CGGTATGGTGAAGGCTGGATTTNCAGGAGATGATGCTCCCAGGGCTGTTTTTCCCAGTNT 270
      || ||||| |||||||||||  | ||||||||||||||||| |   |||| |||||| |

 139 TGGAATGGTTAAGGCTGGATTCGCTGGAGATGATGCTCCCAGAGGGTTTTTCCCCAGTGT 198

 271 TGTTGGTAGGCCAAGACATCATGGTGTCATGGTTGGNATGAACCAGAAGGATGCATATNT 330
     ||||||| | || ||||||||||||||||||||||| ||||| ||||| ||||| ||| |

 199 TGTTGGTCGACCTAGACATCATGGTGTCATGGTTGGGATGAATCAGAAAGATGCGTATGT 258

 331 TGGTGATGAAGCACAATCCANGNGAGGTATTCTTACCTTGAAGNATCCTTTT 382
     ||||||||||||||||||||   ||||||| || || |||||  | ||| ||

 259 TGGTGATGAAGCACAATCCAAAAGAGGTATCCTCACATTGAAATACCCTATT 310

____________________________

Hit Accession Number X17526 originally from EMBL database

Hit Sequence Description :: D.carota RNA for actin gene Ac1.
Length of Hit Sequence = 1177
P-value = 2.500000e-49

HSP Alignments:

 Score: 698  Strand = Plus   Identities = 78%
 157 ATAAAAAATGGCTGAGGCTGATGATATTCAACCAATCGTGTGTGACAATGGTACCGGTAT 216

     | | | ||||||||| | ||| |||||||||||  | || ||||| ||||| || || ||

  27 ACAGACAATGGCTGATGGTGAAGATATTCAACCTCTTGTCTGTGATAATGGAACTGGAAT 86

 217 GGTGAAGGCTGGATTTNCAGGAGATGATGCTCCCAGGGCTGTTTTTCCCAGTNTTGTTGG 276
     ||| |||||||||||| | || ||||||||||| |||||||| ||||||||| |||||||

  87 GGTTAAGGCTGGATTTGCTGGTGATGATGCTCCAAGGGCTGTGTTTCCCAGTATTGTTGG 146

 277 TAGGCCAAGACATCATGGTGTCATGGTTGGNATGAACCAGAAGGATGCATATNTTGGTGA 336
      || ||  ||||   |||||| |||||||| |||  ||| |||||||| ||| |||||||

 147 CAGACCTCGACACACTGGTGTGATGGTTGGAATGGGCCAAAAGGATGCCTATGTTGGTGA 206

 337 TGAAGCACAATCCANGNGAGGTATTCTTACCTTGAAGNATCCTTTT 382
     ||||||||||||||   | |||||  | || |||||  | ||  ||

 207 TGAAGCACAATCCAAAAGGGGTATCATCACATTGAAATACCCAATT 252

____________________________

Length of Hit Sequence = 1384

P-value = 4.500000e-28

HSP Alignments:

 Score: 454  Strand = Plus   Identities = 68%
 173 GCTGATGATATTCAACCAATCGTGTGTGACAATGGTACCGGTATGGTGAAGGCTGGATTT 232

     | ||||||| ||    |  ||||    ||||||||  | ||||||   || ||||| || 

   7 GATGATGATGTTGCCGCTCTCGTCGTAGACAATGGATCTGGTATGTGCAAAGCTGGTTTC 66

 233 NCAGGAGATGATGCTCCCAGGGCTGTTTTTCCCAGTNTTGTTGGTAGGCCAAGACATCAT 292
      | |||||||||||||| || ||||| || ||     | || ||  | ||  | ||||| 

  67 GCTGGAGATGATGCTCCAAGAGCTGTCTTCCCTTCAATCGTAGGACGTCCCCGTCATCAG 126

 293 GGTGTCATGGTTGGNATGAACCAGAAGGATGCATATNTTGGTGATGAAGCACAATCCANG 352
     || ||||||||||| |||   || || ||| ||||  | || |||||||| |||  || |

 127 GGAGTCATGGTTGGTATGGGACAAAAAGATTCATACGTCGGAGATGAAGCTCAAAGCAAG 186

 353 NGAGGTATTCTTACCTTGAAGNATCC 378
      |||||||  | ||||||||  | ||

 187 AGAGGTATCTTGACCTTGAAATACCC 212

Citations and Credits

The script for creating this results file is the work of Libby Shoop.

The BLAST programs whose results are listed here are documented in:

Altschul, Stephen F., Warren Gish, Webb Miller, Eugene W. Myers, and David J. Lipman (1990). Basic local alignment search tool. J. Mol. Biol. 215:403-410.

Back to the Sample TC Report

Sample Output from University of Minnesota's Sequence Analysis Project