LOCUS R65270 411 bp mRNA EST 07-AUG-1995 DEFINITION 13774 Arabidopsis thaliana cDNA clone 168H2T7. ACCESSION R65270 NID g937751 KEYWORDS EST. SOURCE thale cress clone=168H2T7 library=Lambda-PRL2 strain=var columbia vector=lambda Zip-Lox primer=T7 dye primer Rsite1=Sal Rsite2=Not Lambda PRL2 is a cDNA library derived from equal quantities of 4 pools of mRNA. The mRNA sources were 1) 7 day germinated etiolated seedlings; 2) tissue culture grown roots; 3) staged plants half with 24 hour light cycle, half on 16 hr light, 8 hour dark- rosettes; 4) same plants as 3 but aerial tissue (stems, flowers and siliques. The vector is BRL's lambda Zip-Lox. The cDNA inserts were directionally cloned with Sal-Not arms using oligo dT primed cDNA. . ORGANISM Arabidopsis thaliana Eucaryotae; Embryophyta; Magnoliophyta; Magnoliopsida; Capparales; Brassicaceae; Arabidopsis. REFERENCE 1 (bases 1 to 411) AUTHORS Newman,T., de Bruijn,F.J., Green,P., Keegstra,K., Kende,H., McIntosh,L., Ohlrogge,J., Raikhel,N., Somerville,S., Thomashow,M., Retzel,E. and Somerville,C. TITLE Genes galore: a summary of methods for accessing results from large-scale partial sequencing of anonymous Arabidopsis cDNA clones JOURNAL Plant Physiol. 106, 1241-1255 (1994) COMMENT Contact: Thomas Newman MSU-DOE Plant Research Laboratory Michigan State University MSU-DOE-PRL, Michigan State University,Plant Biology Bldg.,E. Lansing,Mi Tel: 517-353-0854 Fax: 517-353-9168 Email: 22313tcn@ibm.cl.msu.edu. NCBI gi: 937751 FEATURES Location/Qualifiers source 1..411 /organism="Arabidopsis thaliana" /clone="168H2T7" /strain="var columbia" /note="thale cress" BASE COUNT 105 a 78 c 97 g 115 t 16 others ORIGIN 1 tcctcacttt catcagccgt tttnaatctc cggcgacttg acagagaaga acaaggaaga 61 agactaagag agaaagtaag agataatcca ggagattcat tctccgtttt naatctncct 121 caatctcatc ttcttccgct cttnctttcc aagctcataa aaaatggctg aggctgatga 181 tattcaacca atcgtgtgtg acaatggtac cggtatggtg aaggctggat ttncaggaga 241 tgatgctccc agggctgttt ttcccagtnt tgttggtagg ccaagacatc atggtgtcat 301 ggttggnatg aaccagaagg atgcatatnt tggtgatgaa gcacaatcca ngngaggtat 361 tcttaccttg aagnatcctt tttgngcatg gngttttnag cacnnggggt g //