NASC

Donated by

• Isobel Parkin Molecular Genetics, Saskatoon Research Centre , Agriculture and Agri-Food Canada
• Steve Robinson Molecular Genetics, Saskatoon Research Centre , Agriculture and Agri-Food Canada

Click here to view all 15647 of these lines.

Description

Saskatoon Arabidopsis T-DNA mutant population - SK collection

A population of almost 50,000 activation tagged A. thaliana lines that have been archived as individual lines to the T3 generation. The population is an excellent tool for both reverse and forward genetic screens and has been used successfully to identify a number of novel mutants. Flanking sequence tags (FST) have been generated and mapped for 15,507 lines to enable further application of the population, while providing a clear distribution of T-DNA insertions across the genome. The population is being screened for a number of biochemical and developmental phenotypes. The population provides an additional tool for plant researcher's to assist with determining gene function for the many as yet uncharacterised genes annotated within the Arabidopsis genome sequence. The presence of enhancer elements on the inserted T-DNA element (pSKI015) allows both knock-out and dominant activation phenotypes to be identified for traits of interest.

References

• Robinson, S.J., et al. 2009. An archived activation tagged population of Arabidopsis thaliana to facilitate forward genetics approaches. BMC Plant Biology 9:101. PMID. 19646253.