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The European Arabidopsis Stock Centre

Isogenic collection of transparent testa (tt) mutants

Donated by

  • Bernd Weisshaar CeBiTec (Center for Biotechnology), Faculty of Biology, Bielefeld University

Click here to view all 26 of these lines.


Isogenic collection of transparent testa (tt) mutants

For further details about this collection see the publication “Update on transparent testa mutants from Arabidopsis thaliana: Characterisation of new alleles from an isogenic collection.” Planta (accepted) authored by Ingo Appelhagen, Katharina Thiedig, Niclas Nordholt, Nina Schmidt, Gunnar Huep, Martin Sagasser, and Bernd Weisshaar.

Proanthocyanidins (PAs) appear brown in their oxidised polymeric forms and accumulate as pigments in A. thaliana seed coats. Mutants impaired in PA accumulation are referred to as transparent testa (tt) or tannin deficient seed (tds). More than 20 mutants of these types have been published, probably representing most of the genes relevant for PA accumulation in A. thaliana. We have performed a systematic phenotypic characterisation of almost all available tt and tds mutants and built a collection of mutants in the genetic background of the accession Columbia to minimise effects arising from ecotype variation.


This collection is available for ordering as individual homozygous lines or as a complete set of 26 lines N2105571.

Nasc code Description Stock contents
N2105571 Complete set of tt-isogenic collection lines View set contents

The collection comprises of the following individual stocks:

NASC Stock Allele AGI code Affected gene Parents line
N2105572 tt4-15 At5g13930 chalcone synthase (CHS) GK-545D04
N2105573 tt4-11 At5g13930 chalcone synthase (CHS) SALK_020583
N2105574 tt5-2 At3g55120 chalcone isomerase (CHI) GK-176H03
N2105575 tt6-2 At3g51240 flavanone 3-hydroxylase (F3H) GK-292E08
N2105576 tt6-5 At3g51240 flavanone 3-hydroxylase (F3H) GK-352E12
N2105577 tt7-6 At5g07990 flavanone 3'hydroxylase (F3'H) GK-349F05
N2105578 tt7-7 At5g07990 flavanone 3'hydroxylase (F3'H) GK-629C11
N2105579 tds4-2 At4g22880 leucoanthocyanidin dioxygenase (LDOX) SALK_028793
N2105580 tds4-4 At4g22880 leucoanthocyanidin dioxygenase (LDOX) SALK_073183
N2105581 ban-5 At1g61720 anthocyanidin reductase (ANR) SALK_040250
N2105582 tt15-3 At1g43620 UGT80B1 (TT15) GK-337G08
N2105583 tt15-4 At1g43620 UGT80B1(TT15) GK-556B06
N2105584 tt15-5 At1g43620 UGT80B1(TT15) GK-016H07
N2105585 aha10-6 At1g17260 autoinhibited H(+)-ATPase 10 (AHA10) GK-170A07
N2105586 tt12-2 At3g59030 MATE family protein TT12 (TT12) GK-797D03
N2105587 tt19-8 At5g17220 glutathione S-transferase 26 (GST26) SALK_105779
N2105588 tt10-7 At5g48100 laccase-like 15 (LAC15) GK-146E10
N2105589 tt10-8 At5g48100 laccase-like 15 (LAC15) GK-026E11
N2105590 tt16-4 At5g23260 agamous-like 32 (AGL32) GK-685G11
N2105591 tt16-5 At5g23260 agamous-like 32 (AGL32) GK-962A05
N2105592 tt1-3 At1g34790 WIP domain protein 1 (TT1) SALK_026171
N2105593 tt2-5 At5g35550 MYB123 (TT2) SALK_005260
N2105594 tt8-6 At4g09820 BHLH042 (TT8) GK-241D05
N2105595 ttg1-21 At5g24520 transparent testa glabra (TTG1) GK-580A05
N2105596 ttg1-22 At5g24520 transparent testa glabra (TTG1) GK-286A06
N2105597 ttg2-5 At2g37260 WRKY44 (TTG2) GK-409B01


  • Appelhagen, I. et al. 2014. Update on transparent testa mutants from Arabidopsis thaliana: characterisation of new alleles from an isogenic collection. Planta 240(5):955-70. PubMed ID: 24903359.