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The European Arabidopsis Stock Centre

Chromosome Substitution Strains

Donated by

  • M.J. Kearsey School of Biosciences, University of Birmingham
  • TimothyMark Wilkes School of Biosciences, University of Birmingham

Click here to view all 4 of these lines.



This resource is comprised of 4 individual homozygous chromosome substitution strains (CSS) for chromosomes 2,3,4 and 5, where a single Col-0 chromosome has been substituted for by the corresponding ler-0 chromosome. These have the names CSS2, CSS3, CSS4, and CSS5.

History of use

These lines were used to produce the STAIRS (STepped Aligned Inbred Recombinant Strains). Single recombinant lines (SRLs) were derived from each of the CSS lines. Each line was backcrossed to Col-0 to produce the F1. This was then Bc to Col-0 to produce SRLs. These were then fixed by selfing.