Ehrhardt fused GFP lines
Donated by
- Sean Cutler Chemical Genomics and Plant Cell Biology, Department of Botany and Plant Sciences, College of Natural and Agricultural Sciences, University of California, Riverside
- David Ehrhardt Department of Plant Biology, The Carnegie Institution of Washington
- Chris Somerville Department of Plant Biology, The Carnegie Institution of Washington
Click here to view all 108 of these lines.
Description
Random GFP::cDNA fusion T-DNA lines
These lines were donated by David Ehrhardt, Sean Cutler and Chris Somerville. They are T-DNA lines for which the transforming vector (pEGAD) includes a CaMV 35S promoter-driven GFP coding region fused to random clones from an Arabidopsis cDNA library. The resulting lines express GFP in varying patterns as influenced by the localization/targeting effects of the gene products of the various cDNAs.
A description of pEGAD and a gallery of images documenting the various patterns of GFP expression among the lines is available here.
Ordering
There are 108 lines, each a product of an independent transformation event using the vector library, which may be ordered as a complete set. In addition, a "Basic Set" of 16 lines has been organized on the basis of varying, patterns of GFP expression. Details are in the table below.
Sets in this collection
| Nasc code | Description | Set contents |
|---|---|---|
| N84722 | Full set of 108 GFP fusion lines; the parental line is included with the set. | View set contents |
| N84723 | Basic set of 16 GFP fusion lines; the parental line is included with the set. | View set contents |
| N84724 | Set of 92 GFP fusion lines not included in the basic set; the parental line is included with the set. | View set contents |