NASC

Donated by

• Nina Fedoroff Department of Biology, Wartik Lab, Eberly College of Science, Pennsylvania State University

Click here to view all 264 of these lines.

Description

Fedoroff T-DNA Collection

This is a collection of 264 transposon T-DNA lines donated by the Fedoroff Transposon Tagging Laboratory.

Transposon T-DNA lines: lines carry a single copy transposon T-DNA (Ds-GUS T-DNA). The transposon is derived from McClintock's maize Activator (Ac) transposable element. This utilizes a beta-glucuronidase (GUS) gene in a promoter/enhancer trap construct and a bacterial aph4 gene (hygromycin resistance) is included as a selectable marker. The transposon is inserted in an herbicide-resistant acetolactate synthase gene, whose function is restored when the transposon excises. The distributed seeds are the R3 generation from a single, homozygous, self-pollinated R2 plant. These can be crossed to Ac stocks N8533-N8538 to induce transposition.

Further Information

Please consult the Plant Transposon Lab web site for a map of the Ds-GUS T-DNA insertions, information on the use of these stocks and results from transposition experiments.

References

• Zhang, S. et al. 2003. Resources for targeted insertional and deletional mutagenesis in Arabidopsis. Plant molecular biology 53(1-2):133-50. PubMed ID: 14756312.