NASC

Donated by

• Patrick Gallois Faculty of Life Sciences, University of Manchester

Click here to view all 283 of these lines.

Description

About the Gallois T-DNA promoter trap lines

This transgenic population contains the promoterless pDGus Bin19 construct donated to Patrick Gallois by Dr Keith Lindsey, Botany Department, Leicester University, U.K. This contains a Kanamycin resistance gene and a promoterless GUS with its ATG next to the T-DNA left border (Topping, J.F. et al. 1991).

Transformation of the plants was carried out by co-cultivation of C24 derived root explants with Agrobacterium tumefaciens followed by selection for transformants on Kanamycin. Seeds were harvested from individual primary transformants (T1). Each progeny was germinated on Kanamycin and resistant plantlets potted out in soil. Seeds were harvested and pooled from plants corresponding to one primary transformant. Seeds from this or the next generation were sent to NASC where they were bulked once more (distribution stocks are therefore either T3 or T4). More than 50% of the lines express GUS in at least one tissue. These lines can be used to identify promoters with specific patterns of expression in the plant.

A general description of the lines may be found in: Devic, M., Hecht, V., Berger, C., Delseny, M. & Gallois, P. 1995. An assessment of promoter trapping as a tool to study plant zygotic embryogenesis. C.R. Acad. Sci. Life Science 318: 121-128.

These lines have been screened for GUS expression in roots and for wavy root mutations by: P.H. Masson (Madison, USA). Described in Rutherford, R., Gallois, P. & Masson, P.H. 1998. Mutations in Arabidopsis thaliana genes involved in the tryptophane biosynthesis pathway affect root waving on tilted agar surfaces. The Plant Journal 16:145-154. PMID. 9839461.

There are 283 individual Gallois promoter trap lines available.

Sets in this collection

Nasc code	Description	Set contents
N5150	Complete set of Gallois lines. Promoter trap population which contains the promoterless pDGus Bin19 construct from Dr. Keith Lindsey; a promoterless GUS with its ATG next to the T-DNA left border.	View set contents

References

• Lindsey, K., et al. 1993. Tagging genomic sequences that direct transgene expression by activation of a promoter trap in plants. Transgenic Research 2(1):33-47. PMID: 8513337.
• Rutherford, R., Gallois, P. & Masson, P.H. 1998. Mutations in Arabidopsis thaliana genes involved in the tryptophan biosynthesis pathway affect root waving on tilted agar surfaces. The Plant journal 16(2):145-54. PMID: 9839461.
• Devic, M. et al. 1995. An assessment of promoter trapping as a tool to study plant zygotic embryogenesis. C.R. Acad. Sci. Paris 318:121-128.