JIC SM lines
Donated by
- Kanu Patel Department of Disease and Stress Biology, John Innes Centre
- Sylvestre Marillonet The Sainsbury Laboratory, John Innes Centre
Click here to view all 26007 of these lines.
Description
The SLAT collection uses the En/Spm element, transposase and positive/negative selection system contained within a single T-DNA, allowing selection to be performed simultaneously without the need for crossing transformants. Plants from the collection have to be sprayed with BASTA in order to select for plants inheriting the transposon. Transposants are stable as the T-DNA will not be present in the progeny due to selection with the counter-selectable marker SU1.
The original SLAT collection contained 48 000 lines in pools of 50. Approximately 12 000 pools were generated and divided up as follows: 48 pools of 50 were assigned to a superpool; a total of 25 superpools were assembled. Nomenclature of the pooled SINS dspm sequences follows the "superpool:pool" pattern such that 01:03 represents Superpool 1: pool 3. The pooled lines were used to create single seeded decent lines from the SLAT collection (24 plants from each pool), this makes genetic analysis much simpler compared with the pools of 50 plants described previously. 24 000 new single seed lines have been generated from the SLAT collection and assigned unique SM numbers.
A detailed protocol explaining how the SM lines were isolated from the SLAT collection and the iPCR protocol used to amplify and sequence the insertion sites can be found here.
Ordering JIC SM lines
Please note the line name may be in a number of different formats. For example SM_3_17168 and SM.17168 refer to the same line. The important identifier is the final long string of numbers. Any prefix you use in the search will be removed.
References
- Tissier, A.F. et al. 1999. Multiple independent defective suppressor-mutator transposon insertions in Arabidopsis: a tool for functional genomics. The Plant Cell 11(10):1841-52.PubMed ID: 10521516