NASC

Donated by

• Don Grierson Plant and Crop Sciences Division, Plant Sciences Building, School of Biosciences, University of Nottingham
• Silin Zhong Plant and Crop Sciences Division, Plant Sciences Building, School of Biosciences, University of Nottingham

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Description

Fluorescent tagging using pGreen vectors

These vectors were developed for use in fluorescent protein tagging. They are suitable for FRET, BiFC and localization studies. Three latest bright fluorescent protein variants (Cerulean, EGFP, Venus) were incorporated into these vectors (see reference below).

18 GATEWAY compatible binary vectors based on the pGreen backbone were developed for Agrobacterium-mediated transformation. These constructs carry one of the three most frequently used plant marker genes (kanamycin, hygromycin and BASTA). One advantage of pGreen compared to the traditional binary vectors is that it requires a helper plasmid pSOUP for propagation in Agrobacterium. Thus, plasmid size is kept at minimum and they can be easily modified in E coli.

5 vectors based on pDH51 were generated for transient expression. They contain only the 35S promoter, GATEWAY cassette, fluorescent protein tag and 35S terminator. The small size of these plamids enables efficient DNA coating in biolistic transformation using less DNA.

References

• Zhong, S. et al. 2008. Improved plant transformation vectors for fluorescent protein tagging. Transgenic Research 17(5): 985-989.PubMed ID: 18594998.