NASC

Donated by

• Venkatesan Sundaresan Department of Plant Biology, College of Biological Sciences, University of California, Davis
• Bindu Nishal Department of Plant Biology, College of Biological Sciences, University of California, Davis
• Titima Tantikanjana Institute of Molecular Agrobiology, National University of Singapore

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Description

Sundaresan heat shock lines

Lines generated by the system of inducible insertional mutagenesis based on the Ac-Ds family of transposons for targeted tagging in Arabidopsis. The Ac and Ds elements were placed in the same T-DNA construct and a heat shock-inducible transposase fusion was utilized to control the levels of transposase gene expression, generating a transposition that can be subsequently stabilized without requiring crossing or segregation. These were mapped by thermal asymmetric interlaced-PCR. Lines can be used as potential launch pad for heat shock mutagenesis; Kanamycin resistant (50 mg/L).

References

• Nishal, B., Tantikanjana, T. & Sundaresan, V. 2005. An inducible targeted tagging system for localized saturation mutagenesis in Arabidopsis. Plant physiology 137(1):3-12.PubMed ID: 15644463.