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The European Arabidopsis Stock Centre

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Search result for '27946 '. Viewing records 1801 to 1850 of 2372 hits.



N868546 Name: SAIL_891_A12.C Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868550 Name: SAIL_894_C07.C Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868552 Name: SAIL_894_H03.C Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868556 Name: SAIL_896_E09.C Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868558 Name: SAIL_896_G07C1 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868564 Name: SAIL_899_H07.C Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868570 Name: SAIL_902_D01.C Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868571 Name: SAIL_902_E03 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868583 Name: SAIL_910_C02.C Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868586 Name: SAIL_913_C08.C Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868588 Name: SAIL_914_B04.C Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868592 Name: SAIL_916_A10.C Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868601 Name: SAIL_1056_F12C1 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868603 Name: SAIL_1057_H07C Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868605 Name: SAIL_1142_E09C Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868622 Name: SAIL_1153_A08C Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868626 Name: SAIL_1157_B07 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868627 Name: SAIL_1158_H11C Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868628 Name: SAIL_1163_B05C Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868634 Name: SAIL_1169_G02C Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868635 Name: SAIL_1170_C06C Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SAIL line, homozygous for the insertion.
N868660 Name: SALK_015176 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion.
N868688 Name: SALK_028216 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion.
N868866 Name: SALK_101672 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion.
N868902 Name: SALK_119327 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion.
N868915 Name: SALK_126859 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion.
N868936 Name: SALK_137440 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion.
N869012 Name: SALK_067276 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion.
N869172 Name: SALK_106337.1 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869194 Name: SALK_106711.1 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869203 Name: SALK_106863.1 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869215 Name: SALK_107434.1 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869285 Name: SALK_109499.0 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869294 Name: SALK_109669.0 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869304 Name: SALK_109794.1 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869321 Name: SALK_110475.0 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869407 Name: SALK_113053.0 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869418 Name: SALK_113168.0 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869422 Name: SALK_113182.1 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869434 Name: SALK_113315.0 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869450 Name: SALK_113493.1 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869500 Name: SALK_114410.1 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869503 Name: SALK_114488.1 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Locus Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869511 Name: SALK_114624.2 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869532 Name: SALK_115166.0 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869578 Name: SALK_115706.0 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869590 Name: SALK_116352.1 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869623 Name: SALK_117658.2 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869632 Name: SALK_117901.2 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.
N869674 Name: SALK_118764.0 Price: £11.00
Donor
  • The Salk Institute for Biological Studies Joseph R. Ecker
Stock type: individual line
Material type: seed


Description
confirmed line isolated from original SALK line, homozygous for the insertion. T-DNA insertions are identified by TDNA-Seq, a method for next-generation sequencing in which pools of T-DNA lines can be sequenced in parallel and then de-convoluted based on their pooling pattern to assign T-DNA insertions back to their originating seed line. To set up the pools, a large numbers of individual TDNA insertion seed lines (10,000) are given a unique four digit number (0000-9999). Based on this identifier a line is placed into one of ten pools (1-10) in each of four superpool sets (plate, row, column and number). All 40 pools (4 superpools x 10 pools) are separately sequenced using a method to enrich for T-DNA/genomic junctions. A T-DNA identified in all four superpools is the unique identifier of the seed line which contained that insert.