NASC

N799366

Name: secGFP line S76 (1)

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description secGFP line S76 (1). Secreted myc-tagged mGFP5 expressed from pVKHEn6-secGFP. Hygromycin resistant.

N799367

Name: secGFP line S76 (2)

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description secGFP line S76 (2). A second family from the same transformant as tube 1.

N799368

Name: GFP-HDEL line H13

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description GFP-HDEL line H13. ER resident mGFP5-ER from Jim Haselloff expressed from pVKHEn6-GFP-HDEL. Hygromycin resistant.

N799369

Name: GFP-HDEL line H8

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description GFP-HDEL line H8. ER resident mGFP5-ER from Jim Haseloff expressed from pVKHEn6-GFP-HDEL. Hygromycin resistant.

N799370

Name: secRFP line 4B8-2

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description secRFP line 4B8-2. Secreted mRFP1 expressed from pVKHEn6-secRFP. Hygromycin resistant. The utility of this construct is that it retains fluorescence in the cell wall (and vacuole if you send it there; Samalova et al., (2006) Traffic 7, 1701-1723). Hygromycin resistant.

N799371

Name: nlsRm-2A-secGf line 74-6

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description nlsRm-2A-secGf line 74-6. Secreted flag-tagged mGFP5 expressed in stoichiometric quantities with a nuclear myc-tagged mRFP1 from pVKHEn6- nlsRm-2A-secGf. The mRFP1 can be used to normalise for expression level between individual plants or cells. Hygromycin resistant.

N799372

Name: nlsRm-2A-secGf line 74-10

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description nlsRm-2A-secGf line 74-10. A second family from the same transformant as Tube 6.

N799373

Name: nlsRm-2A-GH line 20-1

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description nlsRm-2A-GH line 20-1 ER-localised mGFP5 expressed in stoichiometric quantities with a nuclear mRFP1 from pVKHEn6- nlsRm-2A-GH. The mRFP1 can be used to normalise for expression level between individual plants or cells. Acts as a reference point for the secGFP construct. Hygromycin resistant.

N799374

Name: nlsRm-2A-GH line 21-1

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description nlsRm-2A-GH line 21-1. An independent transgenic line with the same construct as Tube 8.

N799375

Name: N-ST-YFP Line T418-1

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description N-ST-YFP Line T418-1. N-glycosylated Golgi-localised YFP based on N-ST-GFP expressed from pVKHEn6-ST-GFP. Hygromycin resistant.

N799376

Name: ST-RFP Line 6-1

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description ST-RFP Line 6-1. Golgi-localised mRFP1 based on ST-GFP expressed from pVKHEn6-ST-RFP. Plasmid constructed by Federica Brandizzi?s lab. Hygromycin resistant.

N799377

Name: N-YFP-HDEL Line T2-3

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description N-YFP-HDEL Line T2-3. N-glycosylated ER-resident YFP expressed from pVKHEn6-N-YFP-HDEL. Hygromycin resistant.

N799378

Name: ST-RFP (see tube 11) in gnomlike1-2 (gnl1-2)

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description ST-RFP (see tube 11) in gnomlike1-2 (gnl1-2).

N799379

Name: ST-RFP (see tube 11) in gnomlike1-1 (gnl1-1)

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description ST-RFP (see tube 11) in gnomlike1-1 (gnl1-1). The gnl1-1 allele was provided by Gerd Jurgens and Sandra Richter, University of Tubingen.

N799380

Name: PMA4-GFP

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description PMA4-GFP. GFP-tagged Nicotiana plumbaginifolia PM H+-ATPase, PMA4, expressed from pVKHen6-PMA4-GFP. Labels the plasma membrane and is incortporated into BFA compartments. Hygromycin resistant.

N799381

Name: PMA4-GFP (see tube 15) in gnomlike1-2 (gnl1-2)

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description PMA4-GFP (see tube 15) in gnomlike1-2 (gnl1-2).

N799382

Name: N-YFP-HDEL (see tube 12) in gnomlike1-2 (gnl1-2)

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description N-YFP-HDEL (see tube 12) in gnomlike1-2 (gnl1-2).

N799383

Name: aleu-GFP

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description aleu-GFP. mGFP6 with signal peptide and sequence-specific vacuolar sorting signal from Petunia aleurain. Labels PVC and vacuolar lumen but is unstable in the lumen unless the plants are grown in the dark.

N799384

Name: Nag-EGFP

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description Nag-EGFP. EGFP fused to N-acetylglucosamine transferase I. labels Golgi stacks. PPT-resistant. From Markus Grebe.

N799385

Name: Nag-EGFP (see tube 19) in gnomlike1-2 (gnl1-2)

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description Nag-EGFP (see tube 19) in gnomlike1-2 (gnl1-2).

N799386

Name: YFP:RAB-E1d

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description YFP:RAB-E1d . YFP-tagged Arabidopsis thaliana RAB-E1d expressed from pVKHEn6-RAB-E1d. Hygromycin resistant.

N799387

Name: YFP:RAB-E1d (see tube 21) in gnomlike1-2 (gnl1-2)

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description YFP:RAB-E1d (see tube 21) in gnomlike1-2 (gnl1-2).

N799388

Name: N-YFP-HDEL (see tube 12) in gnomlike1-1 (gnl1-1)

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description N-YFP-HDEL (see tube 12) in gnomlike1-1 (gnl1-1). The gnl1-1 allele was provided by Gerd Jurgens and Sandra Richter, University of Tubingen.

N799389

Name: GNL1-YFP. YFP-tagged GNOMLIKE1

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description GNL1-YFP. YFP-tagged GNOMLIKE1 in secGFP background (see Tube 1). Kanamycin and hygromycin resistant. The secGFP marker is frequently silenced in this line. The GNL1-YFP plasmid was provided by Gerd Jurgens and Sandra Richter, University of Tubingen.

N799390

Name: GNL1-YFP (see tube 24) in gnomlike1-2 (gnl1-2)

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description GNL1-YFP (see tube 24) in gnomlike1-2 (gnl1-2).

N799391

Name: GNL1(L696M)-YFP. YFP-tagged brefeldinA-sensitive GNOMLIKE1 mutant (L696M)

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description GNL1(L696M)-YFP. YFP-tagged brefeldinA-sensitive GNOMLIKE1 mutant (L696M) in secGFP background (see Tube 1). Kanamycin and hygromycin resistant. The secGFP marker is frequently silenced in this line. The GNL1-YFP plasmid was provided by Gerd Jurgens and Sandra Richter, University of Tubingen.

N799392

Name: ST-N-Rm-2A-secGf line 39-1

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description ST-N-Rm-2A-secGf line 39-1. Secreted flag-tagged mGFP5 expressed in stoichiometric quantities with an N-glycosylated Golgi-localised myc-tagged mRFP1 from pVKHEn6-ST-N-Rm-2A-secGf. Owing to a vacuolar sorting determinant in the 2A peptide sequence that remains attached to the mRFP1 marker, this marker traffics from the Golgi to the vacuole where it accumulates. Hygromycin resistant.

N799393

Name: ST-N-Rm-2A-secGf line 48-1

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description ST-N-Rm-2A-secGf line 48-1. An independent transgenic line with the same construct as Tube27.

N799394

Name: ST-N-Rm-2A-GH line 54-4

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description ST-N-Rm-2A-GH line 54-4. ER-localised mGFP5 expressed in stoichiometric quantities with an N-glycosylated Golgi-localised myc-tagged mRFP1 from pVKHEn6-ST-N-Rm-2A-secGf. Owing to a vacuolar sorting determinant in the 2A peptide sequence that remains attached to the mRFP1 marker, this marker traffics from the Golgi to the vacuole where it accumulates. Hygromycin resistant.

N799395

Name: ST-N-Rm-2A-GH line 59-2

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description ST-N-Rm-2A-GH line 59-2. An independent transgenic line with the same construct as Tube 29.

N799396

Name: sec-N-Rm-2A-secGf line 39-1

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description sec-N-Rm-2A-secGf line 39-1. Secreted flag-tagged mGFP5 expressed in stoichiometric quantities with an N-glycosylated vacuole-localised myc-tagged mRFP1 from pVKHEn6-ST-N-Rm-2A-secGf. Owing to a vacuolar sorting determinant in the 2A peptide sequence that remains attached to the mRFP1 marker, this marker traffics via the Golgi and PVC to the vacuole where it accumulates. Hygromycin resistant.

N799397

Name: sec-N-Rm-2A-secGf line 48-1

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description sec-N-Rm-2A-secGf line 48-1. An independent transgenic line with the same construct as Tube 31.

N799398

Name: sec-N-Rm-2A-GH line 54-4

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description sec-N-Rm-2A-GH line 54-4. ER-localised mGFP5 expressed in stoichiometric quantities with an N-glycosylated vacuole-localised myc-tagged mRFP1 from pVKHEn6-ST-N-Rm-2A-secGf. Owing to a vacuolar sorting determinant in the 2A peptide sequence that remains attached to the mRFP1 marker, this marker traffics via the Golgi and PVC to the vacuole where it accumulates. Hygromycin resistant.

N799399

Name: sec-N-Rm-2A-GH line 59-2

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description sec-N-Rm-2A-GH line 59-2. An independent transgenic line with the same construct as Tube 33.

N799400

Name: gnl1-3

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description gnl1-3 homozygous F4 seed from second backcross; line 1.

N799401

Name: gnl1-3

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description gnl1-3 homozygous F4 seed from second backcross; line 2.

N799402

Name: N799402

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description Dexamethasone-inducible over-expression of Arabidopsis wild-type RAB-A2a using expression vector pH-TOP in the 35S:LhGR driver line 4C-S5/7. Tubes 37 and 38 are lines 1.45 and 1.33 respectively. T2 seed. The pH-TOP:RAB-A2a T-DNA is hygromycin resistant (but weak) and segregating; the 35S:LhGR T-DNA is kanamycin resistant and homozygous.

N799403

Name: N799403

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description Dexamethasone-inducible over-expression of Arabidopsis wild-type RAB-A2a using expression vector pH-TOP in the 35S:LhGR driver line 4C-S5/7. Tubes 37 and 38 are lines 1.45 and 1.33 respectively. T2 seed. The pH-TOP:RAB-A2a T-DNA is hygromycin resistant (but weak) and segregating; the 35S:LhGR T-DNA is kanamycin resistant and homozygous.

N799404

Name: N799404

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description Dexamethasone-inducible over-expression of S26N mutant of Arabidopsis RAB-A2a using expression vector pH-TOP in the 35S:LhGR driver line 4C-S5/7. Tubes 39 and 40 are lines 1.33 and 1.69 respectively. The pH-TOP:RAB-A2a T-DNA is hygromycin resistant (but weak) and segregating; the 35S:LhGR T-DNA is kanamycin resistant and homozygous.

N799405

Name: N799405

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description Dexamethasone-inducible over-expression of S26N mutant of Arabidopsis RAB-A2a using expression vector pH-TOP in the 35S:LhGR driver line 4C-S5/7. Tubes 39 and 40 are lines 1.33 and 1.69 respectively. The pH-TOP:RAB-A2a T-DNA is hygromycin resistant (but weak) and segregating; the 35S:LhGR T-DNA is kanamycin resistant and homozygous.

N799406

Name: N799406

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description Dexamethasone-inducible over-expression of Q71L mutant of Arabidopsis RAB-A2a using expression vector pH-TOP in the 35S:LhGR driver line 4C-S5/7. Tubes 41 and 42 are lines 1.3 and 1.9 respectively. The pH-TOP:RAB-A2a T-DNA is hygromycin resistant (but weak) and segregating; the 35S:LhGR T-DNA is kanamycin resistant and homozygous.

N799407

Name: N799407

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description Dexamethasone-inducible over-expression of Q71L mutant of Arabidopsis RAB-A2a using expression vector pH-TOP in the 35S:LhGR driver line 4C-S5/7. Tubes 41 and 42 are lines 1.3 and 1.9 respectively. The pH-TOP:RAB-A2a T-DNA is hygromycin resistant (but weak) and segregating; the 35S:LhGR T-DNA is kanamycin resistant and homozygous.

N799408

Name: N799408

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description Dexamethasone-inducible over-expression of N125I mutant of Arabidopsis RAB-A2a using expression vector pH-TOP in the 35S:LhGR driver line 4C-S5/7. Tubes 43 and 44 are lines 1.5 and 1.7 respectively. The pH-TOP:RAB-A2a T-DNA is hygromycin resistant (but weak) and segregating; the 35S:LhGR T-DNA is kanamycin resistant and homozygous.

N799409

Name: N799409

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description Dexamethasone-inducible over-expression of N125I mutant of Arabidopsis RAB-A2a using expression vector pH-TOP in the 35S:LhGR driver line 4C-S5/7. Tubes 43 and 44 are lines 1.5 and 1.7 respectively. The pH-TOP:RAB-A2a T-DNA is hygromycin resistant (but weak) and segregating; the 35S:LhGR T-DNA is kanamycin resistant and homozygous.

N799410

Name: YFP:RAB-A2a

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description YFP:RAB-A2a. YFP(Venus) inserted at the initiation codon of a genomic fragment encompassing the Arabidopsis RAB-A2a locus. Tubes 45 and 46 are lines 2.1 and 1.5 respectively and are derived from two independently constructed plasmids. These lines were used for the analysis in Figure 5 of Chow et al. (2008) Plant Cell 20, p101-123. Kanamycin resistant T2 seed; segregating.

N799411

Name: YFP:RAB-A2a

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description YFP:RAB-A2a. YFP(Venus) inserted at the initiation codon of a genomic fragment encompassing the Arabidopsis RAB-A2a locus. Tubes 45 and 46 are lines 2.1 and 1.5 respectively and are derived from two independently constructed plasmids. These lines were used for the analysis in Figure 5 of Chow et al. (2008) Plant Cell 20, p101-123. Kanamycin resistant T2 seed; segregating.

N799412

Name: YFP:RAB-A2a[S26N]

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description YFP:RAB-A2a[S26N]. YFP(Venus) inserted at the initiation codon of a genomic fragment encompassing the Arabidopsis RAB-A2a locus carrying the S26N mutation. Tubes 47, 48 and 49 are lines 3.6, 2.5, and 2.4 respectively and are derived from two independently constructed plasmids. These lines were used for the analysis in Figure 5 of Chow et al. (2008) Plant Cell 20, p101-123. Kanamycin resistant T2 seed; segregating.

N799413

Name: YFP:RAB-A2a[S26N]

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description YFP:RAB-A2a[S26N]. YFP(Venus) inserted at the initiation codon of a genomic fragment encompassing the Arabidopsis RAB-A2a locus carrying the S26N mutation. Tubes 47, 48 and 49 are lines 3.6, 2.5, and 2.4 respectively and are derived from two independently constructed plasmids. These lines were used for the analysis in Figure 5 of Chow et al. (2008) Plant Cell 20, p101-123. Kanamycin resistant T2 seed; segregating.

N799414

Name: YFP:RAB-A2a[S26N]

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description YFP:RAB-A2a[S26N]. YFP(Venus) inserted at the initiation codon of a genomic fragment encompassing the Arabidopsis RAB-A2a locus carrying the S26N mutation. Tubes 47, 48 and 49 are lines 3.6, 2.5, and 2.4 respectively and are derived from two independently constructed plasmids. These lines were used for the analysis in Figure 5 of Chow et al. (2008) Plant Cell 20, p101-123. Kanamycin resistant T2 seed; segregating.

N799415

Name: YFP:RAB-A2a[Q71L]

Price: £11.00

Donor University of Oxford Ian Moore

Stock type: individual line

Material type: seed

Description YFP:RAB-A2a[Q71L]. YFP(Venus) inserted at the initiation codon of a genomic fragment encompassing the Arabidopsis RAB-A2a locus carrying the Q71L mutation. Tubes 50, 51 and 52 are lines 2.8, 11.7, and 2.1 respectively and are derived from two independently constructed plasmids. These lines were used for the analysis in Figure 5 of Chow et al. (2008) Plant Cell 20, p101-123. Kanamycin resistant T2 seed; segregating.