NASC

N4004

Name: School Teaching Kit

Price: £0.00

Donor Wageningen University Maarten Koornneef

Stock type: set

Material type: seed

Description Plant Curiosity Kit - contains NW20, NW30, N31, NW41, N45, N64. This plant 'curiosity' kit is intended to encourage students to appreciate that mutant plants are interesting. The contents are a mix of seeds from several strikingly different Arabidopsis mutants. These can be planted out in the school or home and will grow in a matter of weeks to show variability. They will readily produce viable seeds that can then be replanted to demonstrate inheritance.

N16562

Name: Education kit -Gibberellic Acid Physiology and Plant Transformation

Price: £0.00

Donor Arabidopsis Biological Resource Center

Stock type: set

Material type: seed

Description This set of three seed stocks and two DNA stocks can be used with the "Gibberellic Acid Physiology and Plant Transformation" module to introduce GA (gibberellic acid) hormone physiology and plant transformation in an undergraduate laboratory module. In this module, Arabidopsis ga5 semi-dwarf mutant plants with reduced activity of GA 20-oxidase are transformed with a construct containing the GA5 gene. The dwarf mutant plants transformed with a wild-type version of the gene show normal size, demonstrating successful complementation of the mutant phenotype. Besides visual observation of the phenotype, students learn additional techniques, including PCR and agarose gel electrophoresis. This experiment is cost effective and can be completed within a 4-month term.

N19982

Name: Education kit

Price: £0.00

Donor Wageningen University Maarten Koornneef University of California, Davis John Bowman

Stock type: set

Material type: seed

Description This set of 3 stocks are used in the module "RECONSTRUCTING THE EVOLUTION OF CAULIFLOWER AND BROCCOLI". Students will experience and understand one approach to studying the evolution of development. Students will apply their understanding of nucleic acids, proteins, transcription factors, development, evolutionary selection, and the species concept to a specific problem: the origin of domesticated cauliflower and broccoli. Students will develop an understanding of basic flowering plant morphology. Students will make and test hypotheses about a mutant CAULIFLOWER (CAL) gene using gene sequence information from different Brassica oleracea subspecies. Students will gain bioinformatics skills working with gene and protein sequences from a public database.

N19983

Name: Education Kits: Same Genes, Different Fates

Price: £0.00

Donor University of North Carolina, Chapel Hill Alan M. Jones University of North Carolina, Chapel Hill Amber Vogel

Stock type: set

Material type: seed

Description kit contents are sufficient for 24 students working in groups of four; to reconstitute GUS substrate (provided in a 15 ml tube wrapped in foil) add 10 drops of rubbing alcochol, mix vigorously and fill the tube up to the 10 ml mark with tap water; detailed instructions for the preparation and storage of the GUS substrate can be found in the Same Genes, Different Fates handout provided at ABRC Education Website.

N19984

Name: Play Mendel Basic

Price: £0.00

Donor Arabidopsis Biological Resource Center

Stock type: set

Material type: seed

Description This set of two stocks can be used with the "Play Mendel Basic" module to guide students through the process of investigating reference and mutant strains of Arabidopsis to learn about Mendelian genetics. Through these activities students will learn about concepts such as growth and development, anatomy, variation, segregation, inheritance, phenotypes and genotypes.

Phenotype no phenotype information available at this time

N19985

Name: Education kit

Price: £0.00

Donor Wageningen University Maarten Koornneef University of Chicago Justin Borevitz

Stock type: set

Material type: seed

Description This set of 4 seed stocks (NW20, N25, N28169, N28175) is used in the "PLAY MENDEL" module, a part of ABRC "Greening the Classroom" Teaching Modules. Students are first introduced to the causal relationship of genetic mutation and morphological variation by growing Arabidopsis plants with different genetic backgrounds ("wild type" and mutant) in their classrooms. Next, they follow and analyze inheritance and segregation of a trait (e.g. floral mutations, leaf hairs) to understand concepts central to Mendelian genetics.

N19986

Name: Education kit

Price: £0.00

Donor The Carnegie Institution of Washington Chris Somerville The Carnegie Institution of Washington Shauna Somerville

Stock type: set

Material type: seed

Description This set of 2 seed stocks (N907, N210) is used to test mutant (pgm-1) and wild-type (Col) response to gravity, described in the publication "How Can Plants Tell Which Way Is Up?". The set of lab exercises described in this publication enables students to understand the basis of gravitropic response using starch synthesis mutant. It also introduces scientific concepts such as hypothesis testing and other statistical analyses.

N19987

Name: Education kit

Price: £0.00

Donor Wageningen University Maarten Koornneef University of California, Riverside Jian-Kang Zhu Monsanto Carrie Schneider University of California, Santa Barbara Ruth Finkelstein

Stock type: set

Material type: seed

Description This set of 10 seed stocks (NW20, N21, N22, N24, N3862, N6252, N22574, N28722, N28828 and N70000) is used in the "THINK GREEN" module, a part of ABRC "Greening the Classroom" Teaching Modules. In this module, students will investigate how plant genotype and natural variation influence plant responses to environmental conditions and how this affects survival in a changing environment.

N19988

Name: Education kit

Price: £0.00

Donor Wageningen University Maarten Koornneef Michigan State University Jan Zeevaart

Stock type: set

Material type: seed

Description This set of 5 stocks is used in the LIFE IN BLOOM module, a part of ABRC Greening the Classroom Teaching Modules. Students will compare the germination rate of mutants defective in the early (ga1) and late (ga5) steps of gibberellic acid (GA) biosynthesis with the corresponding wild type (non-mutant), Ler, as well as comparing a mutant in GA sensing and response (gai-1). By comparing germination rates and totals in seeds grown in water or GA solution under light or dark conditions, students will gain an understanding of the requirement for GA in germination. They will also observe how supplying exogenous GA can reverse the phenotypes of some mutants, but not of others.

N19990

Name: Education kit

Price: £0.00

Donor University of Wisconsin, Madison Michael Sussman The Plant Gene Expression Center Sarah Hake University of Delaware Allan Shapiro University of Delaware Chu Zhang

Stock type: set

Material type: seed

Description This set of 11 seed stocks (N25261, N16358, N25262, N3763, N6141, N6357, N6497, N70756, N70764, N8849 & N8851) is used to demonstrate use of native promoter-driven reporter gene expression. Students will be able to observe distinct Beta-glucuronidase (GUS) expression patterns determined by the promoters of 11 genes with a very well-known function. Note that CS6357 and CS16358 represent translational fusions and nine other lines all represent transcriptional fusions.

N19991

Name: Education kit

Price: £0.00

Donor The Ohio State University Emma Knee

Stock type: set

Material type: seed

Description This set of 4 stocks (NW20, N6213, N6219, N6224) is used in the "WHO TURNED OUT THE LIGHTS?" module. Students will learn how plants sense differences in their light environment through the action of photoreceptors and respond by modifying their growth and development. The set includes wild type (non-mutant) Ler seeds, phytochrome A and phytochrome B mutant seeds, and double mutant seed in which both photoreceptors are mutated in the same plant.

N19992

Name: Education kit

Price: £0.00

Donor The Ohio State University Luz Rivero

Stock type: set

Material type: seed

Description This set of 5 stocks (N70000, N76390, N76412, N76384, N76387) is used in the module "Germination". These natural variants, including a lab strain and four locally adapted lines from different climates, illustrate the variation in light and temperature requirements for germination in Arabidopsis. Students will germinate seeds in light and darkness following a 3 day cold (stratification) period or without stratification and observe differences in germination in the different natural variants.

N19993

Name: CS19993

Price: £0.00

Donor Pace University Eric Brenner

Stock type: set

Material type: seed

Description This set of three stocks can be used with the "Regulation of Lateral Root Growth" module to introduce students to the usefulness of reporter constructs in transgenic systems to study gene expression. Students will learn the role of auxin in the stimulation of genes that lead to the formation of lateral roots using GUS staining. Contains 4 contiguous lab sessions.

Phenotype no phenotype information available at this time

N19994

Name: Education kits

Price: £0.00

Donor John Innes Centre Caroline Dean John Innes Centre Clare Lister

Stock type: set

Material type: seed

Description This set of 53 stocks can be used with the PlantingScience module "Genetics in Inbred Arabidopsis", a 4-8 week module for upper high school courses such as Genetics, Biotechnology, Honors or AP Biology. This module is also well suited for undergraduate courses. In this module, which was designed as a whole-class experiment for students with a basic biology and genetics background, students work in teams to understand genotypic variation among recombinant inbred lines (RILs) of the model organism Arabidopsis thaliana. Seeds are used from two parent lines, Columbia and Landsberg, as well as numerous recombinant inbred lines (RILs) developed by crossing the parent lines. In a guided inquiry investigation, students sow and cultivate all the plants in the same controlled environments (to minimize variation due to environment), and monitor traits to observe genetic variation among the RILs. Protocols are provided to investigate whether given traits are continuous (quantitative) traits - the result of multiple genes, or if they are discrete traits - the result of a single gene. In addition to this guided inquiry, students may also choose other traits to investigate. Student teams pool data with other teams in the class to analyze trends and patterns, such as identifying continuous versus discrete traits, and determining potential genetic linkages among traits. Students communicate with scientist mentors online to discuss their experiment, from generating questions to constructing evidence-based conclusions. Set includes 51 RILs and 2 parents.

N19995

Name: Education kit

Price: £0.00

Donor University of Georgia Susan Wessler

Stock type: set

Material type: seed

Description TRANSPOSING FROM THE LABORATORY TO THE CLASSROOM. The module is a modified version of a published experiment where students experience how virtual transposable elements from rice (Oryza sativa) are assayed for function in transgenic Arabidopsis thaliana. As part of the module, students analyze the phenotypes and genotypes of transgenic plants to determine the requirements for transposition. After mastering the skills and concepts, students participate in an authentic research project where they use computational analysis and PCR to detect transposable element insertion site polymorphism in a panel of diverse maize strains.

N19996

Name: Education kit

Price: £0.00

Donor St. Louis Community College Elizabeth Boedeker St. Louis Community College Eilene Lyons

Stock type: set

Material type: seed

Description This set of 2 stocks (NW20, N290) is used in the module "Molecular Genotyping of Arabidopsis". The goal of this laboratory is to introduce students to the action of transposons, plant DNA extraction and the use of polymerase chain reaction (PCR) to compare diploid genotypes. A transposon insertion is identified in curly leaf (clf-2) mutant and Ler wild-type is used as a control.

N19997

Name: Education Kits:

Price: £0.00

Donor University of Western Ontario Kim Loney

Stock type: set

Material type: seed

Description This set of 2 stocks is used in the module EXPRESSION ANALYSIS OF LIGHT-REGULATED GENES IN THE det1-1 MUTANT. The module is based on light signaling pathways in plants, specifically Arabidopsis. Over the course of three labs, students are introduced to the techniques of RNA isolation and Reverse Transcription PCR (RT-PCR) for the purpose of analyzing differences in mRNA content of light-regulated genes between wild-type and det1-1 mutant samples. First, students are able to assess phenotypic differences between wild-type and det1-1 plants grown in the presence or absence of light. Next, through RT-PCR students gain an understanding of how mutation in one gene can cause downstream effects in signaling pathways to alter the response to light.Donors: Kim Loney.

N19998

Name: Education kit

Price: £0.00

Donor University of Pennsylvania Scott Poethig University of Pennsylvania Linda Robinson

Stock type: set

Material type: seed

Description This set of 2 stocks (N70000, N6955) can be used to generate F2 plants used in the module "Genetics of Arabidopsis thaliana". In this module students use F2 plants of a dihybrid cross between gl1-1 hy4-B104 (cry1) double mutant and Col wild-type to follow the segregation of two independent traits as well as to explore the relationship between genotype and phenotype using molecular methods. Recommended for use in college-level courses.

N19999

Name: Education kit

Price: £0.00

Donor Ohio University Sarah Wyatt

Stock type: set

Material type: seed

Description This set of 4 stocks (N2360, N67144, N67145, N67146) is used to demonstrate a range of altered responses to gravity of shoots and hypocotyls at room temperature after gravistimulation at 4C. It contains Ws wild type and gps mutants: no response (gps1), wrong way (gps2) and over achiever (gps3). GPS1 is a cytochrome P450 subunit, the identity of GPS2 and GPS3 is not known.

N20000

Name: Education kit

Price: £0.00

Donor Ohio University Sarah Wyatt

Stock type: set

Material type: seed

Description This set of 8 GFP seed lines (N9210, N9217, N9244, N9245, N9247, N9249, N9251, N9266) from J. Haseloff and screened by Sarah Wyatt has been identified as a plant anatomy set for use in undergraduate / beginning graduate courses; this set includes lines expressing GFP in roots, shoots and flowers.

N27962

Name: Genetics of Floral Development

Price: £0.00

Donor Emory & Henry College Christine Fleet

Stock type: set

Material type: seed

Description This set of ten stocks can be used to demonstrate concepts presented in the Teaching Tools in Plant Biology module "Genetics of Floral Development". This module explores the ABCDE model of floral organ development.

Phenotype no phenotype information available at this time

N27963

Name: Introduction to Phytohormones

Price: £0.00

Donor Emory & Henry College Christine Fleet Harvey Mudd College Mary Williams University of Maryland Caren Chang Tohoku University Shinjiro Yamaguchi

Stock type: set

Material type: seed

Description This set of ten stocks can be used to demonstrate concepts presented in the following Teaching Tools in Plant Biology modules: Introduction to Phytohormones, The Story of Auxin, Abscisic Acid, Brassinosteriods, Cytokinins, Ethylene, Gibberellins, and Strigolactones. By growing and observing this set of stocks students will see how mutations affecting hormone pathways and signaling can affect the phenotype of resulting plants.

N27964

Name: Leaf Development 2 (Genetic Control of Leaf Development)

Price: £0.00

Donor University of Cologne John Chandler Harvey Mudd College Mary Williams

Stock type: set

Material type: seed

Description This kit can be used to demonstrate the concepts presented in the Teaching Tools in Plant Biology module "Leaf Development 2 (Genetic Control of Leaf Development)". This module explores the genetic controls that influence how a leaf is formed. The ten stocks included in this kit include seven mutants with their corresponding reference strains. By growing and observing this set of seeds students will see how mutations can affect various areas of leaf development including identity, polarity, size and shape, and differentiation.

N27965

Name: Plant Curiosity Advanced module

Price: £0.00

Donor Arabidopsis Biological Resource Center Nottingham Arabidopsis Stock Centre

Stock type: set

Material type: seed

Description This mix of seeds, which includes six strains of Arabidopsis, can be used with the Plant Curiosity Advanced module to demonstrate a variety of science concepts. By observing the growth of this set of seeds, students will see how different mutations can affect the phenotype of Arabidopsis plants. This module requires 19 weeks for completion of all of the planting procedures and assignments. A shortened version of this module is available as the Plant Curiosity Basic module (CS4004), which requires seven weeks for completion.

N27966

Name: Molecular Control of Shoot Architecture

Price: £0.00

Donor The University of São Paulo Lazaro Eustaquio Pereira Peres

Stock type: set

Material type: seed

Description This set of ten stocks can be used to demonstrate the concepts presented the Teaching Tools in Plant Biology module “Molecular Control of Shoot Architecture”. This module explores plant growth, development and pattern generation. The ten stocks in this kit include one natural accession, and seven mutants with their corresponding reference strains. By growing and observing this set of seeds, students will see the effect of various mutations on plant development.

N27971

Name: CS27971 Education kit

Price: £0.00

Donor Wageningen University Maarten Koornneef Michigan State University Jan Zeevaart

Stock type: set

Material type: seed

Description This set of 2 stocks is used in the LIFE IN BLOOM - BASIC module. Students will compare the germination rate of mutants defective in the early (ga1) step of gibberellic acid (GA) biosynthesis with the corresponding wild type (non-mutant). By comparing germination rates and totals in seeds grown in water or GA solution, students will gain an understanding of the requirement for GA in germination. They will also observe how supplying exogenous GA can reverse the phenotypes of the ga1 mutant.

N27972

Name: Understanding the Influence of Mutations on Protein Structure and Function

Price: £0.00

Donor University of Radford Tara Phelps-Durr

Stock type: set

Material type: seed

Description This set of three stocks can be used with the "Understanding the Influence of Mutations on Protein Structure and Function" module available through CourseSource. In this module, computational molecular modeling is used to demonstrate how DNA mutations cause phenotypes. ASYMMETRIC LEAVES1 and 2 (AS1 and AS2) mutants affecting leaf development are used as an example of this link. The module includes the computational modeling of the AS1 and AS2 normal and mutant proteins. Recommended for Sophomore/Junior Undergraduate level.

Phenotype no phenotype information available at this time