NASC

N21

Name: abscisic acid deficient

Price: £11.00

Donor Wageningen University Maarten Koornneef

Locus aba1

Photo

Stock type: individual line

Material type: seed

Description Hormone

Phenotype aba deficient; brown-yellow leaves, non-dormant seeds, germinate in silique on plant at high relative humidity, wilty, reduced vigor.

N22

Name: abscisic acid insensitive

Price: £11.00

Donor Wageningen University Maarten Koornneef

Locus abi1

Stock type: individual line

Material type: seed

Description Hormone

Phenotype dominant mutation, increased resistance to ABA (10 microM), young plants wilt readily.

N23

Name: abscisic acid insensitive

Price: £11.00

Donor Wageningen University Maarten Koornneef

Locus abi2

Stock type: individual line

Material type: seed

Description Hormone

Phenotype abscisic acid resistant, young plants wilt readily.

N24

Name: abscisic acid insensitive

Price: £11.00

Donor Wageningen University Maarten Koornneef

Locus abi3

Stock type: individual line

Material type: seed

Description Hormone

Phenotype abscisic acid resistant, acts in same transduction pathway as ABI4 and ABI5; reduced dormancy.

NW58

Name: GA deficient

Price: £11.00

Donor Wageningen University Maarten Koornneef

Locus ga1

Photo

Stock type: individual line

Material type: seed

Description Hormone

Phenotype Gibberellin responsive dwarf.

N59

Name: GA deficient

Price: £11.00

Donor Wageningen University Maarten Koornneef

Locus ga2

Stock type: individual line

Material type: seed

Description Hormone

Phenotype Gibberellin responsive dwarf.

N60

Name: GA deficient

Price: £11.00

Donor Wageningen University Maarten Koornneef

Locus ga3

Stock type: individual line

Material type: seed

Description Hormone

Phenotype Gibberellin responsive dwarf.

N61

Name: GA deficient

Price: £11.00

Donor Wageningen University Maarten Koornneef

Locus ga4

Stock type: individual line

Material type: seed

Description Hormone

Phenotype Gibberellin responsive dwarf.

N62

Name: GA deficient

Price: £11.00

Donor Wageningen University Maarten Koornneef

Locus ga5

Stock type: individual line

Material type: seed

Description Hormone

Phenotype Gibberellin responsive dwarf.

NW63

Name: GA insensitive dwarf

Price: £11.00

Donor Wageningen University Maarten Koornneef

Locus gai

Photo

Stock type: individual line

Material type: seed

Description Hormone

Phenotype Dominant mutation. Dark green leaved dwarf.

N155

Name: abscisic acid deficient

Price: £11.00

Donor Wageningen University Maarten Koornneef

Locus aba1

Stock type: individual line

Material type: seed

Description Hormone

Phenotype reduced seed dormancy; wilty (esp. in low relative humidity); slightly darker green plants; less vigorous than wild type.

N156

Name: abscisic acid deficient

Price: £11.00

Donor Wageningen University Maarten Koornneef

Locus aba2

Stock type: individual line

Material type: seed

Description Hormone

Phenotype reduced seed dormancy; wilty (esp. in low relative humidity); slightly darker green plants; less vigorous than wild type.

N157

Name: abscisic acid deficient

Price: £11.00

Donor Wageningen University Maarten Koornneef

Locus ABA3

Stock type: individual line

Material type: seed

Description Hormone

Phenotype reduced seed dormancy; slightly wilty; high ABA (abscisic acid) concentration; slightly darker green, less vigorous than wild type.

N158

Name: abscisic acid deficient

Price: £11.00

Donor Wageningen University Maarten Koornneef

Locus aba3

Stock type: individual line

Material type: seed

Description Hormone

Phenotype reduced seed dormancy; wilty, especially in low relative humidity; slightly darker green plants; less vigorous than wild type; salt tolerant; seeds germinate and seedlings turn green on 200 mM NaCl.

N225

Name: glutathione up-regulated

Price: £11.00

Donor Leiden University Bert J. van der Zaal

Locus gl1 GUP1

Stock type: individual line

Material type: seed

Description hormone, biochemical

Phenotype Both gup1-1 and gup2-1 (stocck number N226) have no clear phenotype when grown in soil. When grown in vitro, both exhibit epinastic growth of leaves. Root growth is slightly more sensitive to IAA inhibition of elongation. Both gup1-1 and gup2-1 lead to enhanced kanamycin resistance in the AIR1-8 background via an nptII gene under control of a tobacco GST promoter . gup1-1 also shows an increased mRNA level of GST5 from Arabidopsis (designated At103-1a in van der Kop et. al. 1999).

N226

Name: glutathione up-regulated

Price: £11.00

Donor Leiden University Bert J. van der Zaal

Locus gl1 GUP2

Stock type: individual line

Material type: seed

Description hormone, biochemical

Phenotype Both gup1-1 (stock number N225) and gup2-1 have no clear phenotype when grown in soil. When grown in vitro, both exhibit epinastic growth of leaves. Root growth is slightly more sensitive to IAA inhibition of elongation. Both gup1-1 and gup2-1 lead to enhanced kanamycin resistance in the AIR1-8 background via an nptII gene under control of a tobacco GST promoter . gup1-1 also shows an increased mRNA level of GST5 from Arabidopsis (designated At103-1a in van der Kop et. al. 1999).

N228

Name: ACC insensitive

Price: £11.00

Donor Ghent University Dominique Van Der Straeten

Locus ain1

Stock type: individual line

Material type: seed

Description Biochemical, hormone

Phenotype Acc insensitive; large rosettes, delayed in bolting. Generally similar to W.T.

N229

Name: ACC insensitive

Price: £11.00

Donor Ghent University Dominique Van Der Straeten

Locus ain1

Stock type: individual line

Material type: seed

Description Biochemical, hormone

Phenotype Acc insensitive; large rosettes, delayed in bolting. Generally similar to W.T.

N237

Name: Ethylene insensitive

Price: £11.00

Donor Wageningen University Maarten Koornneef

Locus etr1

Stock type: individual line

Material type: seed

Description Hormone

Phenotype Dominant mutation, root and hypocotyl elongation insensitive to ethylene, leaf chlorophyll content not reduced in ethylene, reduced ethylene binding activity, large rosette, delayed in bolting, allelic to ein1-1.

N3070

Name: ethylene insensitive

Price: £11.00

Donor The Salk Institute for Biological Studies Joseph R. Ecker

Locus etr1

Photo

Stock type: individual line

Material type: seed

Description hormone, form,

Phenotype ethylene insensitive; root and hypocotyl elongation insensitive to ethylene, leaf chlorophyll content not reduced in ethylene, reduced ethylene binding activity; large rosettes, delayed in bolting.

N3071

Name: ethylene insensitive

Price: £11.00

Donor The Salk Institute for Biological Studies Joseph R. Ecker

Locus ein2

Photo

Stock type: individual line

Material type: seed

Description hormone, flowering

Phenotype ethylene insensitive; large rosettes, delayed in bolting.

N3074

Name: auxin resistant

Price: £11.00

Donor Indiana University Mark Estelle

Locus aux1

Photo

Stock type: individual line

Material type: seed

Description Hormone

Phenotype aerial portions of plant similar in appearance to wild type; slight increase in root elongation and altered geotropic response; resistant to ethylene and auxin.

N3075

Name: axr1-3

Price: £11.00

Donor Indiana University Mark Estelle

Locus axr1

Photo

Stock type: individual line

Material type: seed

Description Hormone

Phenotype Auxin resistant. Irregular rosette leaves, tend to curl downward; short petioles; slightly reduced plant height; increased number of lateral branches (ca. 2x).

N3076

Name: axr1-12

Price: £11.00

Donor Indiana University Mark Estelle

Locus axr1

Photo

Stock type: individual line

Material type: seed

Description Hormone

Phenotype Auxin resistant. Irregular rosette leaves (same as axr1-3 except more pronounced); plant height reduced to less than half of wild type; inflorescences more highly branched; reduced fertility.

N3077

Name: auxin resistant

Price: £11.00

Donor Indiana University Mark Estelle

Locus axr2

Photo

Stock type: individual line

Material type: seed

Description Hormone

Phenotype dark green, dwarf; small, round rosette leaves, short petioles; inflorescences curved, twisted, and shorter than wild type; roots have altered gravitropic response, lacking root hairs; resistant to ethylene, abscisic acid and auxin.

N3103

Name: gibberellin deficient

Price: £11.00

Donor Michigan State University Jan Zeevaart

Locus ga1

Stock type: individual line

Material type: seed

Description Hormone

Phenotype gibberellin responsive dwarf; small, dark green leaves; requires gibberellin for germination and seed set.

N3104

Name: gibberellin deficient

Price: £11.00

Donor Michigan State University Jan Zeevaart

Locus ga1

Stock type: individual line

Material type: seed

Description Hormone

Phenotype gibberellin responsive dwarf; small, dark green leaves; requires gibberellin for germination and seed set.

N3105

Name: gibberellin deficient

Price: £11.00

Donor Michigan State University Jan Zeevaart

Locus ga1

Stock type: individual line

Material type: seed

Description Hormone

Phenotype gibberellin responsive dwarf; small, dark green leaves; requires gibberellin for germination and seed set.

N3106

Name: gibberellin deficient (leaky)

Price: £11.00

Donor Michigan State University Jan Zeevaart

Locus ga1

Photo

Stock type: individual line

Material type: seed

Description Hormone

Phenotype leaky mutant; semi-dwarf; germinates, flowers, and produces seeds without gibberellin treatment.

N3107

Name: gibberellin deficient (leaky)

Price: £11.00

Donor Michigan State University Jan Zeevaart

Locus ga1

Photo

Stock type: individual line

Material type: seed

Description Hormone

Phenotype leaky; more dwarf than ga1-5; germinates without, but seed production enhanced by, gibberellin sprays.

N3817

Name: auxin resistant

Price: £11.00

Donor Indiana University Mark Estelle Adelphi University Lawrence Hobbie

Locus AXR6

Stock type: individual line

Material type: seed

Description hormone, embryo, form,

Phenotype Phenotype of homozygote: embryonic development is characterized by aberrant patterns of cell division, leading to defects in the cells of the suspensor, root and hypocotyl precursors, and provasculature; growth of seedlings arrests soon after germination; seedlings lack a root and hypocotyl and display severe vascular pattern defects in their cotyledons; usually possess one cotyledon or occasionally two, attached to a small basal stub. Phenotype of heterozygote: rosette leaves are shorter and more wrinkled than wt; plants are short and bushy (have increased number of basal inflorescences); fertile and green; siliques are at a more acute angle relative to the stem than wt; seedlings are auxin resistant in root and hypocotyl elongation, have delayed root gravity response and decreased number of lateral roots; backcrossed 6 times.

N3818

Name: auxin resistant

Price: £11.00

Donor Indiana University Mark Estelle Adelphi University Lawrence Hobbie

Locus AXR6

Stock type: individual line

Material type: seed

Description hormone, embryo, form

Phenotype phenotype of homozygote: embryonic development is characterized by aberrant patterns of cell division, leading to defects in the cells of the suspensor, root and hypocotyl precursors, and provasculature; growth of seedlings arrests soon after germination; seedlings lack a root and hypocotyl and display severe vascular pattern defects in their cotyledons; usually with two cotyledon although sometimes with one or with several small true leaves, joined to a small basal stub. Phenotype of heterozygote: less severely affected in all aspects of phenotype than axr6-1; rosette leaves are short and wrinkled; plants are short and somewhat bushy (have increased number of basal inflorescences); fertile and green; siliques are at a more acute angle relative to the stem than wt; seedlings are auxin resistant in root and hypocotyl elongation, have delayed root gravity response and decreased number of lateral roots; backcrossed 8 times.

N3834

Name: abscisic acid deficient

Price: £11.00

Donor Rice University Sue Gibson

Locus aba2

Stock type: individual line

Material type: seed

Description Hormone

Phenotype abscisic acid deficient, ABA and dormancy responses appear to be as expected for aba2; reduced sensitivity to the inhibitory effects of sugars on early seedling development; seeds will germinate and form seedlings with expanded cotyledons and true leaves on concentrations of glucose (0.3M), sucrose (0.3M) and sorbitol (0.4 M) that inhibit these processes in wt plants; very slightly decreased sensitivity to mannose (2mM) on germination and early seedling development; decreased sensitivity to the presence of the GA biosynthesis inhibitor paclobutrazol (35 mg/L); increased rate of water loss (leaf transpiration), which leads to symptoms of wilting and withering especially under low relative humidity and water stress; reduced growth, reduced plant size and vigor; allelic to sis4-1.

N3835

Name: abscisic acid deficient

Price: £11.00

Donor Rice University Sue Gibson

Locus aba2

Stock type: individual line

Material type: seed

Description Hormone

Phenotype abscisic acid deficient, ABA and dormancy responses appear to be as expected for aba2; reduced sensitivity to the inhibitory effects of sugars on early seedling development, seeds will germinate and form seedlings with expandedcotyledons and true leaves on concentrations of glucose (0.3M), sucrose (0.3M) and sorbitol (0.4 M) that inhibit these processes in wt plants; very slightly decreased sensitivity to mannose (2mM) on germination and early seedling development; decreased sensitivity to the presence of the GA biosynthesis inhibitor paclobutrazol (35 mg/L); increased rate of water loss (leaf transpiration), which leads to symptoms of wilting and withering especially under low relative humidity and water stress; reduced growth, reduced plant size and vigor; same as sis4-2.

N3836

Name: abscisic acid insensitive

Price: £11.00

Donor Rice University Sue Gibson

Locus abi4

Stock type: individual line

Material type: seed

Description Hormone

Phenotype abscisic acid resistant, reduced sensitivity to ABA inhibition of germination, germinates in the presence of 5 microM ABA; dormancy response appears to be as expected for abi4; reduced sensitivity to the inhibitory effects of sugars on early seedling development, germinates and forms seedlings with expanded cotyledons and true leaves on concentrations of glucose (0.3 M), sucrose (0.3 M), mannose (2mM) or sorbitol (0.4 M) that inhibit these processes in wt plants; decreased sensitivity to the presence of the GA biosynthesis inhibitor paclobutrazol (35 mg/L ); does not exhibit compromised water relations, not wilty; lacks trichomes on stems and leaves, some trichomes in leaf margins; same as sis5-1.

N3837

Name: abscisic acid insensitive

Price: £11.00

Donor Rice University Sue Gibson

Locus abi4

Stock type: individual line

Material type: seed

Description Hormone

Phenotype abscisic acid resistant, reduced sensitivity to ABA inhibition of germination, germinates in the presence of 5 microM ABA; dormancy response appears to be as expected for abi4; reduced sensitivity to the inhibitory effects of sugars on early seedling development, germinates and forms seedlings with expanded cotyledons and true leaves on concentrations of glucose (0.3 M), sucrose (0.3 M) or sorbitol (0.4 M) that inhibit these processes in wt plants; slightly decreased sensitivity to mannose (2mM) on germination and early seedling development; decreased sensitivity to the presence of the GA biosynthesis inhibitor paclobutrazol (35mg/L ); does not exhibit compromised water relations, not wilty; same as sis5-2.

N3838

Name: abscisic acid insensitive

Price: £11.00

Donor Rice University Sue Gibson

Locus abi4

Stock type: individual line

Material type: seed

Description Hormone

Phenotype abscisic acid resistant, reduced sensitivity to ABA inhibition of germination, germinates in the presence of 5 microM ABA; dormancy response appears to be as expected for abi4; reduced sensitivity to the inhibitory effects of sugars on early seedling development, germinates and forms seedlings with expanded cotyledons and true leaves on concentrations of glucose (0.3 M) , sucrose (0.3 M), mannose (2mM) or sorbitol (0.4 M) that inhibit these processes in wt plants; decreased sensitivity to the presence of the GA biosynthesis inhibitor paclobutrazol (35 mg/L ); does not exhibit compromised water relations, not wilty; lacks trichomes on stems and leaves, some trichomes in leaf margins; same as sis5-3.

N3839

Name: abscisic acid insensitive

Price: £11.00

Donor Rice University Sue Gibson

Locus abi4

Stock type: individual line

Material type: seed

Description Hormone

Phenotype abscisic acid resistant, reduced sensitivity to ABA inhibition of germination, germinates in the presence of 5 microM ABA; dormancy response appears to be as expected for abi4; reduced sensitivity to the inhibitory effects of sugars on early seedling development, germinates and forms seedlings with expanded cotyledons and true leaves on concentrations of glucose (0.3 M), sucrose (0.3 M), mannose (2mM) or sorbitol (0.4 M) that inhibit these processes in wt plants; decreased sensitivity to the presence of the GA biosynthesis inhibitor paclobutrazol (35 mg/L ); does not exhibit compromised water relations, not wilty; lacks trichomes on stems and leaves, some trichomes in leaf margins; same as sis5-4.

N3865

Name: ckh1-1

Price: £11.00

Donor Osaka University Tatsuo Kakimoto

Locus ckh1

Stock type: individual line

Material type: seed

Description Form, Hormone

Phenotype increased sensitivity to cytokinin, callus growth in tissue culture exhibit typical cytokinin responses including rapid proliferation and chloroplast development in response to lower levels of cytokinins than in the wild type; short inflorescences and primary roots when grown in soil; backcrossed once to Ler.

N3866

Name: ckh2-1

Price: £11.00

Donor Osaka University Tatsuo Kakimoto

Locus ckh2

Stock type: individual line

Material type: seed

Description Form, Hormone

Phenotype increased sensitivity to cytokinin, callus growth in tissue culture exhibit typical cytokinin responses including rapid proliferation and chloroplast development in response to lower levels of cytokinins than in the wild type; short inflorescences and primary roots when grown in soil; backcrossed once to Ler.

N8018

Name: auxin resistant

Price: £11.00

Donor Adelphi University Lawrence Hobbie

Locus axr4

Photo

Stock type: individual line

Material type: seed

Description Hormone

Phenotype auxin resistant; irregular rosette leaves, slightly curled around leaf axis; roots elongate on auxin-containing medium; defective root gravitropism; reduced number of lateral roots.

N8053

Name: ethylene insensitive

Price: £11.00

Donor The Salk Institute for Biological Studies Joseph R. Ecker

Locus ein4

Photo

Stock type: individual line

Material type: seed

Description Hormone

Phenotype dominant mutation, ethylene insensitive.

N8072

Name: jasmonate resistant

Price: £11.00

Donor University of Nebraska, Lincoln Paul Staswick

Locus jar1

Photo

Stock type: individual line

Material type: seed

Description Hormone

Phenotype reduced inhibition of seedling root growth when grown in agar media containing methyljasmonate; reduced induction of lipoxygenase and vegetative storage proteins by exogenous methyljasmonate; germination of seeds more sensitive to inhibition by abscisic acid.

N8843

Name: auxin resistant, ethylene insensitive

Price: £11.00

Donor Adelphi University Lawrence Hobbie

Locus AUX1 EIN2

Stock type: individual line

Material type: seed

Description hormone, form, flowering

Phenotype double mutant: aux1-7, ein2. ; resistant to auxin and ethylene; large rosette, delayed bolting; altered geotropic response; slight increase in root elongation; independently isolated allele of the EIN2 locus

N8844

Name: ein2

Price: £11.00

Donor Adelphi University Lawrence Hobbie

Locus

Stock type: individual line

Material type: seed

Description independently isolated allele of the EIN2 locus; backcrossed 5 times

Phenotype insensitive to ethylene; large rosette, delayed in bolting

N16562

Name: Education kit -Gibberellic Acid Physiology and Plant Transformation

Price: £0.00

Donor Arabidopsis Biological Resource Center

Stock type: set

Material type: seed

Description This set of three seed stocks and two DNA stocks can be used with the "Gibberellic Acid Physiology and Plant Transformation" module to introduce GA (gibberellic acid) hormone physiology and plant transformation in an undergraduate laboratory module. In this module, Arabidopsis ga5 semi-dwarf mutant plants with reduced activity of GA 20-oxidase are transformed with a construct containing the GA5 gene. The dwarf mutant plants transformed with a wild-type version of the gene show normal size, demonstrating successful complementation of the mutant phenotype. Besides visual observation of the phenotype, students learn additional techniques, including PCR and agarose gel electrophoresis. This experiment is cost effective and can be completed within a 4-month term.

N27963

Name: Introduction to Phytohormones

Price: £0.00

Donor Emory & Henry College Christine Fleet Harvey Mudd College Mary Williams University of Maryland Caren Chang Tohoku University Shinjiro Yamaguchi

Stock type: set

Material type: seed

Description This set of ten stocks can be used to demonstrate concepts presented in the following Teaching Tools in Plant Biology modules: Introduction to Phytohormones, The Story of Auxin, Abscisic Acid, Brassinosteriods, Cytokinins, Ethylene, Gibberellins, and Strigolactones. By growing and observing this set of stocks students will see how mutations affecting hormone pathways and signaling can affect the phenotype of resulting plants.

N72618

Name: 35S::AP1-GR ap1-1 cal-1

Price: £11.00

Donor California Institute of Technology (Caltech) Elliot Meyerowitz

Locus

Stock type: individual line

Material type: seed

Description ap1-1 cal-1 double mutant expressing a fusion protein of AP1 and the hormone-binding domain of the rat glucocorticoid receptor (GR) from the constitutive cauliflower mosaic virus 35S promoter. Basta resistant. Unknown transgene genotype.

Phenotype ap1-1 cal-1 double mutant phenotype (massive over-proliferation of inflorescence-like meristems, leading to a cauliflower-like appearance; delayed flowering and abnormal flowers lacking sepals and petals). Activation of the AP1-GR fusion protein by a single dexamethasone treatment induces synchronous flower development and rescues the organ identity defects of the ap1-1 cal-1 mutant flowers.

N99346

Name: TEC-SCHP-5-1

Price: £30.00

Donor University of California, San Diego Julian Schroeder University of California, San Diego Felix Hauser

Stock type: individual line

Material type: seed

Description This subpool (CS99346) consists of 90 independent transformants expressing artificial microRNAs (amiRNAs) designed to target homologous, possibly functionally redundant genes in forward genetic screens; represents a subpool of TEC amiRNA pool N99431. Note that the larger pool N99431 is recommended for forward genetic screening of many lines. Generated by transforming Col-0 plants with a combination of 20bp and 21bp amiRNA libraries (CD4-62 and CD4-72, respectively) in p35S::amiRNA3 OCS (pPHANTOM vector) targeting transcripts encoding metabolic and other enzymes catalyzing transfer reactions (EC: 2) (TEC); protein family member functional classes: CHO metabolism, glycolysis, gluconeogenesis, TCA cycle, amino acid metabolism, secondary metabolism, hormone metabolism, transferases, lipid metabolism (Mapman); transferase activity (GO:0016740). Basta resistant. 1250 seeds provided per order.

Phenotype Mixture of phenotypes depending on unknown amiRNA expressed.

N99347

Name: TEC-SCHP-5-2

Price: £30.00

Donor University of California, San Diego Julian Schroeder University of California, San Diego Felix Hauser

Stock type: individual line

Material type: seed

Description This subpool (CS99347) consists of 90 independent transformants expressing artificial microRNAs (amiRNAs) designed to target homologous, possibly functionally redundant genes in forward genetic screens; represents a subpool of TEC amiRNA pool N99431. Note that the larger pool N99431 is recommended for forward genetic screening of many lines. Generated by transforming Col-0 plants with a combination of 20bp and 21bp amiRNA libraries (CD4-62 and CD4-72, respectively) in p35S::amiRNA3 OCS (pPHANTOM vector) targeting transcripts encoding metabolic and other enzymes catalyzing transfer reactions (EC: 2) (TEC); protein family member functional classes: CHO metabolism, glycolysis, gluconeogenesis, TCA cycle, amino acid metabolism, secondary metabolism, hormone metabolism, transferases, lipid metabolism (Mapman); transferase activity (GO:0016740). Basta resistant. 1250 seeds provided per order.

Phenotype Mixture of phenotypes depending on unknown amiRNA expressed.