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NASC

The European Arabidopsis Stock Centre

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Search result for '16562 '. Viewing records 1 to 5 of 5 hits.



NW20 Name: Landsberg erecta (Ler-0) Price: £11.00
Donor
  • Wageningen University Maarten Koornneef
Locus Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Description
Line defined as erecta by Will Feenstra. Background line for all mutants produced by Feenstra, Van der Veen and Koornneef at Wageningen University
Phenotype
blunt fruits, short petioles, usually upright stems, compact inflorescence; Ler-0, ecotype derived from Landsberg (La-0), but genetically distinct; carries erecta gene; blunt siliques, upright stems, bunched flowers; common background line. round leaves, short petioles, short pedicels, flowers are clustered at the top of the inflorescence, short and wide siliques with blunt tip, short and upright stems, compact inflorescence, carries erecta gene; height = 20-25 cm.
N62 Name: GA deficient Price: £11.00
Donor
  • Wageningen University Maarten Koornneef
Locus Stock type: individual line
Material type: seed


Description
Hormone
Phenotype
Gibberellin responsive dwarf.
N16549 Name: 35S::GA5 in ga5-1 background Price: £11.00
Donor
  • Michigan State University John Ohlrogge
Stock type: individual line
Material type: seed


Description
The full length GA5 cDNA was cloned into pGEM-T Easy vector (Promega, Madison, WI) and subcloned as a XbaI-SacI fragment into pBI121 binary vector to replace the GUS gene, producing pBI121-GA5 (pBI-GA5). The construct was introduced into Agrobacterium tumefaciens strain C58C1 to transform GA4-sprayed ga5-1 Arabidopsis plants. Starting at 4-weeks and continuing weekly, ga5 plants that were to be transformed were sprayed with an aqueous solution of 10-4 M solution of GA4, containing 5 percent (v/v) ethanol. This step was necessary to overcome dwarfing and ensure fertility. Surface-sterilized T1 seeds of transformed plants were selected on MS medium supplemented with 50 microgram/ml kanamycin. Resistant seedlings were transferred to soil for continued growth and T2 seeds from several individual kanamycin-resistant plants were pooled. Successive generations (T3, T4) were harvested from plants selected on kanamycin.
Phenotype
Dwarf phenotype of ga5-1 plants was rescued by the introduction of a wild-type copy of the gene in the 35S::GA5/ga5-1 transgenic plants.
N2107936 Name: CD3-388 Price: £11.00
Donor
  • California State University Judy Brusslan
Stock type: individual line
Material type: plasmid dna


Description
Cloning vector. Empty plant transformation vector.
N2107937 Name: CD3-1265  Price: £11.00
Donor
  • Michigan State University John Ohlrogge
  • Algoma University Maria Isabel Molina
Stock type: individual line
Material type: bacterial clone


Description
The pBI121 vector was modified by replacing the GUS reporter with the GA5 cDNA. This construct can be used for plant transformation to complement the ga5-1 mutation.