Search result for '2106368 '.
Viewing records 1 to 29 of 29 hits.
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N2106134
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Name: RED TIDE S17 |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
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Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106135
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Name: RED TIDE S18 |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
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|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106136
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Name: RED TIDE S29 |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
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|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106137
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Name: RED TIDE S32 |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
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|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106138
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Name: RED TIDE SUC2 |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
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Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106139
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Name: RED TIDE WOL |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
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|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106140
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Name: RED TIDE SHR |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
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Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106141
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Name: RED TIDE ATHB8 |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
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Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106142
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Name: RED TIDE IAA19 |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
|
|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106143
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Name: RED TIDE DR5 |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
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|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106144
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Name: RED TIDE PIN1 |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
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Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106145
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Name: RED TIDE PIN4 |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
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|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106146
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Name: RED TIDE WER |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
|
|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106147
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Name: RED TIDE EXP7 |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
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Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106148
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Name: RED TIDE PRP3 |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
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Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106149
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Name: RED TIDE IRT1 |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
|
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Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106150
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Name: RED TIDE GL2 |
Price:
£11.00
|
Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
|
|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106151
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Name: RED TIDE CO2 |
Price:
£11.00
|
Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
|
|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106152
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Name: RED TIDE PEP |
Price:
£11.00
|
Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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|
N2106153
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Name: RED TIDE SCR |
Price:
£11.00
|
Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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|
N2106154
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Name: RED TIDE E47 |
Price:
£11.00
|
Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106155
|
Name: RED TIDE UPB1 |
Price:
£11.00
|
Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
|
Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
|
|
N2106156
|
Name: RED TIDE WOX5 |
Price:
£11.00
|
Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
|
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Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106157
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Name: RED TIDE FEZ |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
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Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106158
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Name: RED TIDE UBQ10 |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
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Material type: seed
|
|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106159
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Name: RED TIDE 2x35S |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
|
Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106160
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Name: RED TIDE RCH1 |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
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Stock type: individual line
|
Material type: seed
|
|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106161
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Name: RED TIDE RCH2 |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
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Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
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Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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N2106162
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Name: RED TIDE KN |
Price:
£11.00
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Donor
- Ecole Normale Supérieure de Lyon Yvon Jaillais
- Centre National de la Recherche Scientifique (CNRS) Gregory Vert
|
Locus
|
Stock type: individual line
|
Material type: seed
|
|
Description
Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col 0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on hygromycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.
|
Phenotype
RED TIDE line. Cell type specific promoter expressing a H2B-2xCherry tag in the nucleus
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