NASC

N2106163

Name: BREAK S4

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106164

Name: BREAK S17

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106165

Name: BREAK S18

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106166

Name: BREAK S29

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106167

Name: BREAK S32

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106168

Name: BREAK SUC2

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106169

Name: BREAK WOL

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106170

Name: BREAK SHR

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106171

Name: BREAK ATHB8

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106172

Name: BREAK IAA19

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106173

Name: BREAK DR5

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus synthetic

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106174

Name: BREAK PIN1

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106175

Name: BREAK PIN4

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106176

Name: BREAK PIN7

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106177

Name: BREAK PIN2

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106178

Name: BREAK WER

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106179

Name: BREAK EXP7

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106180

Name: BREAK PRP3

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106181

Name: BREAK CO2

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106182

Name: BREAK PEP

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106183

Name: BREAK SCR

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106184

Name: BREAK E47

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106185

Name: BREAK UPB1

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106186

Name: BREAK WOX5

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106187

Name: BREAK Q12

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106188

Name: BREAK FEZ

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106189

Name: BREAK UBQ10

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106190

Name: BREAK 2x35S

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus Cauliflower mosaic virus promoter

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106191

Name: BREAK KN

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and ACTIN2prom::BirA expressing plants were transformed by dipping. Primary transformants (T1) were selected in vitro on kanamycin. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype BREAK line. Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method

N2106192

Name: LINE-UP SHR

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta). For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype LINE-UP line. Cell type specific gene induction

N2106193

Name: LINE-UP EXP7

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta). For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype LINE-UP line. Cell type specific gene induction

N2106194

Name: LINE-UP FEZ

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta). For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype LINE-UP line. Cell type specific gene induction

N2106195

Name: LINE-UP UBQ10

Price: £11.00

Donor Ecole Normale Supérieure de Lyon Yvon Jaillais Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Locus

Stock type: individual line

Material type: seed

Description Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate (basta). For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Phenotype LINE-UP line. Cell type specific gene induction