NASC Stock Detail Page
NASC ID: N2112625
Name: proUBI10::AtVIH2K219A-D292A::Flag
ABRC stock number: CS2112625
Description: The vectors containing the AtUBI10 promoter (proUBI10), the corresponding AtVIH2 coding sequence, a FLAG tag, the Nos terminator and Fast Red as a fluorescent marker for the seed coat were transformed into A. tumefaciens strain GV3101. Flower dipping was then used to transform 5-week-old plants (Col-0). T1 plants were selected by red fluorescence using a Nikon SMZ18 stereomicroscope equipped with a RFP-B filter. Lines with single T-DNA insertions were selected by the segregation analysis. T3 lines were propagated and harvested for the seed donation.
Donation Date: 2025-04-07
| Donated by: | University of Geneva Felix Rico-Resendiz |
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Genus: Arabidopsis Species: thaliana
Seed type: Transgenic
Background: Col-0
Segregation status: homozygous
Growth requirement: None
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
No visible phenotype, high Pi levels at rosette stage