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NASC

The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N2112626

Name: proUBI10::AtVIH2R372A-H373A::Flag

ABRC stock number: CS2112626

Description: The vectors containing the AtUBI10 promoter (proUBI10), the corresponding AtVIH2 coding sequence, a FLAG tag, the Nos terminator and Fast Red as a fluorescent marker for the seed coat were transformed into A. tumefaciens strain GV3101. Flower dipping was then used to transform 5-week-old plants (Col-0). T1 plants were selected by red fluorescence using a Nikon SMZ18 stereomicroscope equipped with a RFP-B filter. Lines with single T-DNA insertions were selected by the segregation analysis. T3 lines were propagated and harvested for the seed donation.

Donation Date: 2025-04-07

Donated by:  University of Geneva Felix Rico-Resendiz

Stock type: individual line

Material type: seed

Status Price (£)
Available £11.00

Germplasm Info

Genus: Arabidopsis     Species: thaliana

Seed type: Transgenic

Background: Col-0

Segregation status: homozygous

Growth requirement: None

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
  • Vip homolog 2 (VIH2)

Phenotype

Small rosette and low Pi levels