NASC

NASC ID: N529613

Name: SALK_029613

ABRC stock number: SALK_029613

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_029613.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G16750.1 (at chr 5 pos 5509581 (W/5509535-5509581) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 3, R 2, C 5 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO391016.

SALKseq_029613.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G10652.1 and AT1G10657.1=P355 (at chr 1 pos 3528537 (W/3528478-3528537) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 3, R 2, C 5 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO384328.

SALKseq_029613.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G31250.1 (at chr 2 pos 13320079 (W/13320028-13320079) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 3, R 2, C 5 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO387675.

Donation Date: 2001-01-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_029613

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_029613C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G10652 AT1G10657 AT2G31250 At5g16750 At5g16760

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g16760 F5E19.100 L300-5' :gene annotation - At5g16760

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.