NASC Stock Detail Page
NASC ID: N530503
Name: SALK_030503
ABRC stock number: SALK_030503
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_030503.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G03055.2=P355 and AT1G03060.1=UTR3 (at chr 1 pos 712255 (W/712205-712255) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 5, R 2, C 6 N 6. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO350919. SALKseq_030503.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G19580.1 (at chr 2 pos 8474021 (W/8473957-8474021) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 5, R 2, C 6 N 6. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO354261. SALKseq_030503.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G29270.1 (at chr 3 pos 11235751 (W/11235675-11235751) on the TAIR10). The sequences' 1-77 bps mapped on the genome. Pools P 5, R 2, C 6 N 6. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO357590. SALKseq_030503.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G49210.1=P355 and AT3G49220.1=P355 (at chr 3 pos 18242099 (C/18242099-18242177) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 5, R 2, C 6 N 6. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO360936. SALKseq_030503.5 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G36030.1=P355 and AT4G36040.1=UTR3 (at chr 4 pos 17048121 (C/17048121-17048149) on the TAIR10). The sequences' 1-29 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 5, R 2, C 6 N 6. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO364293.
Donation Date: 2001-01-12
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_030503
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_030503C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At2g20580
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.