NASC

NASC ID: N531335

Name: SALK_031335

ABRC stock number: SALK_031335

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

December 2002 TAIR gene annotation - At4g13710 F18A5.100 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_031335.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G13710.1 (at chr 4 pos 7962989 (C/7962989-7963045) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 53-79 bps onto the T-DNA. Pools P 4, R 2, C 7 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO387777.

SALKseq_031335.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G05190 and AT2G05200.1=P355 (at chr 2 pos 1882001 (W/1881945-1882001) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 4, R 2, C 7 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO381080.

SALKseq_031335.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G07560.1=P355 and AT2G07570.1=P355 (at chr 2 pos 3174546 (W/3174468-3174546) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 4, R 2, C 7 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO384430.

Donation Date: 2003-01-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_031335

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662014 (SALK_031335C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G05190 AT2G05200 AT2G07560 AT2G07570 At4g13710

EMBL accession:

BZ592811

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.