NASC

NASC ID: N531372

Name: SALK_031372

ABRC stock number: SALK_031372

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

December 2002 TAIR gene annotation - At4g31050 F6I18.40 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_031372.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G31050.1 (at chr 4 pos 15115288 (W/15115228-15115288) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 3, R 3, C 4 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO357639.

SALKseq_031372.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G40711.1=P355 and AT2G40720.1 (at chr 2 pos 16987230 (W/16987178-16987230) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 3, R 3, C 4 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO350966.

SALKseq_031372.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G51610.1 (at chr 5 pos 20966452 (W/20966415-20966452) on the TAIR10). The sequences' 1-38 bps mapped on the genome, 38-65 bps onto the T-DNA. Pools P 3, R 3, C 4 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO360982.

SALKseq_031372.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G27473.1 (at chr 3 pos 10170412 (C/10170412-10170490) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 3, R 3, C 4 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO354309.

Donation Date: 2003-01-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_031372

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_031372C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G40711 AT2G40720 AT3G27473 AT5G51610 At4g31050

EMBL accession:

BH758815

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.