NASC

NASC ID: N531976

Name: SALK_031976

ABRC stock number: SALK_031976

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_031976.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT3G23580.1 (at chr 3 pos 8462715 (W/8462661-8462715) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 55-79 bps onto the T-DNA. Pools P 2, R 8, C 9 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO354329.

SALKseq_031976.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G30120.1 (at chr 4 pos 14733550 (W/14733500-14733550) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 54-79 bps onto the T-DNA. Pools P 2, R 8, C 9 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO357658.

SALKseq_031976.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT4G12400.1 (at chr 4 pos 7338764 (W/7338724-7338764) on the TAIR10). The sequences' 1-41 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 2, R 8, C 9 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO361003.

Donation Date: 2001-01-12

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_031976

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_031976C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
		RNR2A	AT4G12400 AT4G30120 At3g23580

EMBL accession:

BH753890

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.