NASC

NASC ID: N532040

Name: SALK_032040

ABRC stock number: SALK_032040

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_032040.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G11950.1 (at chr 2 pos 4819911 (C/4819911-4819989) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 7, R 9, C 3 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO407852.

SALKseq_032040.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G36150.1 and AT1G36160.2=P355 (at chr 1 pos 13531365 (W/13531303-13531365) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 7, R 9, C 3 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO404514.

SALKseq_032040.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G13300.1 (at chr 3 pos 4304860 (W/4304782-4304860) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 7, R 9, C 3 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO411200.

SALKseq_032040.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G21620.1 (at chr 3 pos 7611293 (W/7611237-7611293) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 7, R 9, C 3 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO340943.

Donation Date: 2001-01-12

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_032040

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_032040C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G36150 AT1G36160 AT3G13300 AT3G21620 At2g11950

EMBL accession:

ED579386

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At2g11950

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.